Effects of components of the areca nut on tumor cells

• Main Authors: LIU, PANG-YEN, 劉邦衍
• Other Authors: LIN, MEI-HUEI
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/36v6e7

碩士 === 嘉南藥理大學 === 生物科技系 === 105 === We found that the crude extract of areca nut (ANE) can induce autophagic cell death. This activity is located in the 30-100 kDa fraction of ANE, designated as ANE 30-100K. We also noticed that several different cancer cells showed higher resistance against serum starvation and cisplatin (DDP) after treatment of low concentrations of ANE or ANE 30-100K for about three weeks. This study was to verify whether similarly ANE or ANE 30-100K treatment for cancer cells results in better growth in nude mice. CE81T/VGH cells were chosen for its ability to grow in nude mice. Firstly, cell culturing model showed that CE81T/VGH cells exhibited stronger tolerance against serum deprivation and hypoxia conditions after 3-week ANE 30-100K treatment. Furthermore, the autophagy inhibitor, chloroquine (CQ), acted synergistically with DDP to inhibit ANE 30-100K-treated CE81T/VGH cells. On the other hand, CE81T/VGH cells also achieved better growth significantly in nude mice after 3-week ANE or ANE 30-100K treatment. Single reagent treatment with CQ or DDP received similar effects; however, combimed CQ and DDP application resulted in synergistic reduction in tumor sizes. These results suggest that the growth of tumors in AN users may particularly rely on autophagy, which highlights the possible values to include autophagy inhibitors like CQ in future treatment. Finally, the results of our attempt to explore the mechanism of ANE 30-100K were also given in the last section.