Hypolipidemic effects and molecular mechanisms of Mei-Gin formula in high fat/cholesterol diet-induced hyperlipidemia hamsters

• Main Authors: Qian-Hui Zhong, 鍾千慧
• Other Authors: 顏國欽
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/yjqwm2

碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 106 === Prunus mume Sieb. et Zucc. is the deciduous tree of the Rosaceae family. It has antitussive, expectorant, antiemetic, and antipyretic effects in Chinese traditional medicine. It provides many physiological activities, including anti-obesity, anti-oxidation, anti-osteoporosis, hypoglycemic, anti-fatigue, and anti-cancer. Mei-Gin is the concentrated extract of plum fruit. However, the functional components and physiological effects of the plum fruit are destroied during the long time heating process. Therefore, this research used Mei-Gin formulas which are based on Mei-Gin and added different proportions of plum fruit, black garlic, and Mesona procumbens Hemsl. extracts to prepare seven kinds of Mei-Gin formulas. High-fat/cholesterol diet was used to induce hamsters with hyperlipidemia and the potential on hypolipidemia of these formulas was evaluated. The content is divided into two parts: (i) An animal pretest to screen the most potential sample from different Mei-Gin formulas. (ii) An animal test to evaluate the hypolipidemic effect on different doses of Mei-Gin formula 4. The results of animal pretest showed that Mei-Gin formula 4 could decrease the concentration of serum triglyceride, total cholesterol, and LDL-C. Furthermore, it also reduced the accumulation of hepatic total lipid and promoted the excretion of fecal cholesterol. From these results, Mei-Gin formula 4 was the most effective formula on improving hyperlipidemia. The results of animal test showed that treatment by Mei-Gin formula 4 (100 mg/kg b.w.) could significantly reduce the concentration of serum triglyceride, total cholesterol, and malondialdehyde. Stimultaneously, it also decreased the amount of hepatic total lipid and triglyceride. Besides, it was able to increase the release of fecal total lipid. We explored the hepatic tissue genes of hyperlipidemic hamsters and found that Mei-Gin formula 4 not only promoted the expression of LXR α, ABCG5, ABCA1, and CYP51, but also inhibited the expression of ACC, FAS, DGAT-2, HMG-CoA reductase, SREBP-1a, and SREBP-1c. In addition, it increased the richness and diversity of the gut microbiota in hyperlipidemia hamsters. Also, it increased the number of Lactobacillus acidophilus and Bifidobacterium animalis, and reduced Helicobacter pylori abundance, which could improve cholesterol metabolism and inhibited its synthesis to reach hypolipidemic effect. Based on these results, Mei-Gin formula 4 had the function to improve dyslipidemia in high-fat/cholesterol-diet-induced hamsters. It would be used to develop into health food to regulate serum lipid profiles in the future.