Optimizing Measurement and Analysis Techniques for Biological Spectral characteristics

• Main Authors: Tseng, Tien-Kuan, 曾添冠
• Other Authors: Su, Kuan-Wei
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/pakef5

碩士 === 國立交通大學 === 電子物理系所 === 106 === The main goal of this research is to develop a low-cost measuring system which can be popularized to be a houschold apparatus for analyzing optical spectra of biological solutions. Some signal processing and impurity filtering approaches for the measured samples are studied to effectively enhance the sensitivity and resolution of the developed system in detecting the characteristic spectral signals for biological solution. A thorough database for the absorption, fluorescence, and Raman spectra of some common biological solutions, such as anthocyanin, chlorophyll, hemoglobin, oleic acid, that is closely related to our daily life has been built by the developed system. Furthermore, the developed system is further applied for the detection of fecal occult blood. According to the measurement of absorption spectrum for whole blood, the effective sensitivity of the developed system to identify the occult blood signal is about 0.15 μg-Hb/ 1 ml-water. We find that the results of identification for the fecal occult blood in real-patient sample by the proposed method are highly consistent with the results by the conventional immune method in medical examination. We believe it is greatly feasible to further integrate an efficient algorithm into the proposed system to realize the component analysis for the sampling solution by matching the spectral characteristic signals with the spectrum database for some common biosolutions.