| Summary: | 碩士 === 國立中央大學 === 生物醫學工程研究所 === 106 === In this work, we developed a magnetic field microplatform for observing the cell migration in real time. Meanwhile, the biological effects of Nasopharyngeal carcinoma cancer cells exposured under the high gradient magnetic field (MF) was investigated. The micoplatform was designed with Length = 3.5 cm, Width = 3.5 cm, Height = 1 cm, respectively. Helmholtz Coil and Halbach Arrays were utilized for fabricating a uniform magnetic field and a gradient magnetic field. The system was operated with a field strength between 1.55-70 mT, which was provided by a magnetic array. Most important of all, it is necessary to provide the appropriate environment with the high magnetic field intensity and high gradient magnetic field for cell culture. Given that the living cells were grown in a humidified atmosphere at 37 ℃, where the temperature is a critical factor for culture. Hence, we propose the derivative equation using Ohmic Law for calculating the critical values of temperature immediately. For Halbach Array group, the gradient magnetic field in comparsion with the uniform magnetic field shows the population of S phase of the Nasopharyngeal carcinoma cancer cells increase owing to magnetic stimuli.
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