Study of probiotic potentials of Bacillus licheniformis CK1 and Lactobacillus reuteri Pg4

• Main Authors: Tzu-Hsuan Li, 李姿璇
• Other Authors: 劉嚞睿
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/36e3ph

碩士 === 國立臺灣大學 === 生物科技研究所 === 106 === The research related to probiotics has primarily been focused on improving intestine health, including the maintenance of the intestinal microbiota and modulation of host intestinal barrier integrity. As reported, by feeding with Bacillus licheniformis CK1, the reproductive disorder of pig caused by zearalenone toxin can be ameliorated. The other putative probiotic, like Lactobacillus reuteri Pg4, has been demonstrated to thicken the mucus layer and increase the height of microvilli, indicating that Pg4 enhances the function of host intestinal barrier. However, the detail of mechanisms for CK1 and Pg4 regulation in the barrier function remains unclear. Here, we showed that CK1 and Pg4 possess different probiotic properties, which are related to inhibit the adhesive ability of pathogens and enhance the barrier function of intestinal cells, respectively. These results indicate that CK1 reduces the amounts of pathogens, including Salmonella enterica serovar Typhimurium and Escherichia coli O157：H7, binding to the epithelial cells. Furthermore, Pg4 elevates the expression of ZO-1 and occludin, which contributes to the stability of intestinal monolayer and prevents the disruption from pathogen infection. In this study, we demonstrated that how CK1 and Pg4 are likely to function with epithelial monolayer model, and the competitive interaction with pathogen. In conclusion, both CK1 and Pg4 have their own novel properties for helping intestinal cells. Therefore, CK1 and Pg4 provide a perspective of combination of these probiotics which may apply in human dietary supplement or animal feed additives.