| Summary: | 碩士 === 國立臺灣科技大學 === 機械工程系 === 106 === A tangent vector estimator extended from the 4-pixel 8-connected Freeman chain code is used to fit the curve of DNA image taken by AFM in this research. Persistence length is a kind of physical property that is able to represent the rigidity of molecule chain of DNA. Precise contour length and accurate contour tangent are both required for estimation of accurate persistence length. However, because of the different image resolutions, the calculation of the contour tangent will have the error. In order to eliminate the contour tangent error of the curve of the DNA image, an estimation method of contour tangent for digitized images is developed in this study. Using this method can effectively eliminate the error of contour tangent which caused by the image resolution, and measure the precise contour tangent.
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