Co-localization of the astrocytic proteins Mts1 and clusterin in CNS injury

• Main Author: Augustsson, Mirja
• Format: Others
• Language: English
• Published: Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi 2005
• Subjects: astrocytes; clusterin; GFAP; Mts1; parkin; spinal cord injury; astrocytic cultures; Ca-binding protein; chaperon; ABC; Cell and Molecular Biology; Cell- och molekylärbiologi
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6147

In the case of injury to the CNS, different proteins act to repair and protect cells in the brain and spinal cord. In the present study, we looked at dorsal root injury and hypoglossal nerve avulsion and transection. Here we studied for the first time the expression of Parkin in these types of injuries. However the antibodies against Parkin used here have not been able to detect Parkin in the injuries examined, neither with fluorescence or using DAB. The roles of Mts1, GFAP, and clusterin after injury have been investigated earlier, but their co-localization in the same cells was first shown in this study in the hypoglossal nucleus with immunohisto-chemical methods. These results may also be of value in the process of finding an effective treatment for neurodegenerative disorders such as ALS.