Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry
Separation of complex biological samples is major challenge in bioanalytical chemistry. Ion mobility spectrometry (IM) is an emerging analytical method which provides an additional method to separate structurally similar molecules prior to mass analysis. In particular, the addition of ion mobility t...
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ndltd-VANDERBILT-oai-VANDERBILTETD-etd-03222018-1551332018-03-28T06:02:59Z Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry Dodds, James Nicholas Chemistry Separation of complex biological samples is major challenge in bioanalytical chemistry. Ion mobility spectrometry (IM) is an emerging analytical method which provides an additional method to separate structurally similar molecules prior to mass analysis. In particular, the addition of ion mobility to mass spectrometry provides the ability to resolve isomeric species in complex mixtures, which is a growing challenge in terms of relating structural molecular specificity to biological relevance. This chiral recognition is particularly important in many biological sciences, including translation of amino acids and pharmaceutical production. Utilizing recent advances in ion mobility-mass spectrometry (IM-MS), this work details the empirical underpinnings of separation science from lenses of chromatography, ion mobility, and mass spectrometry. The advances made in these studies will further the ability to distinguish and characterize molecules in complex samples in order to provide increased structural confidence in molecular identification. John A. McLean Steven D. Townsend David E. Cliffel Erin C. Rericha VANDERBILT 2018-03-27 text application/pdf http://etd.library.vanderbilt.edu/available/etd-03222018-155133/ http://etd.library.vanderbilt.edu/available/etd-03222018-155133/ en unrestricted I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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Chemistry Dodds, James Nicholas Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry |
description |
Separation of complex biological samples is major challenge in bioanalytical chemistry. Ion mobility spectrometry (IM) is an emerging analytical method which provides an additional method to separate structurally similar molecules prior to mass analysis. In particular, the addition of ion mobility to mass spectrometry provides the ability to resolve isomeric species in complex mixtures, which is a growing challenge in terms of relating structural molecular specificity to biological relevance. This chiral recognition is particularly important in many biological sciences, including translation of amino acids and pharmaceutical production. Utilizing recent advances in ion mobility-mass spectrometry (IM-MS), this work details the empirical underpinnings of separation science from lenses of chromatography, ion mobility, and mass spectrometry. The advances made in these studies will further the ability to distinguish and characterize molecules in complex samples in order to provide increased structural confidence in molecular identification. |
author2 |
John A. McLean |
author_facet |
John A. McLean Dodds, James Nicholas |
author |
Dodds, James Nicholas |
author_sort |
Dodds, James Nicholas |
title |
Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry |
title_short |
Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry |
title_full |
Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry |
title_fullStr |
Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry |
title_full_unstemmed |
Investigation of Separation Strategies for Bioanalytical Methods: Chromatography, Ion Mobility and Mass Spectrometry |
title_sort |
investigation of separation strategies for bioanalytical methods: chromatography, ion mobility and mass spectrometry |
publisher |
VANDERBILT |
publishDate |
2018 |
url |
http://etd.library.vanderbilt.edu/available/etd-03222018-155133/ |
work_keys_str_mv |
AT doddsjamesnicholas investigationofseparationstrategiesforbioanalyticalmethodschromatographyionmobilityandmassspectrometry |
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1718617522986549248 |