The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets

The normal behavior of Evans blue in the vacuolar apparatus of rat kidney and the changes in this normal behavior imposed by a magnesium deficiency were investigated. Since Evans blue binds tightly to serum albumin, and since Evans blue-albumin complexes are reabsorbed from the glomerular filtrate b...

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Main Author: Seignious, George Williams
Other Authors: Biochemistry and Nutrition
Format: Others
Language:en
Published: Virginia Polytechnic Institute and State University 2016
Subjects:
Online Access:http://hdl.handle.net/10919/70557
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spelling ndltd-VTETD-oai-vtechworks.lib.vt.edu-10919-705572021-05-05T05:40:48Z The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets Seignious, George Williams Biochemistry and Nutrition LD5655.V855 1973.S43 The normal behavior of Evans blue in the vacuolar apparatus of rat kidney and the changes in this normal behavior imposed by a magnesium deficiency were investigated. Since Evans blue binds tightly to serum albumin, and since Evans blue-albumin complexes are reabsorbed from the glomerular filtrate by pinocytosis by the cells of the renal proximal tubules, Evans blue served as a suitable marker of the vacuolar apparatus for these studies. Rats were injected intravenously with Evans blue and sacrificed at either 1/2, 1, 3, or 12 hours post-injection. Kidneys were removed and homogenized. By fractional centrifugations at the different time periods post-injection, the net uptake of Evans blue by the total kidney and each subcellular fraction was observed. The amount of Evans blue taken up by the total kidney was shown to increase with time. Also a gradual increase in the amount of Evans blue in the nuclear fraction with time was seen. This was interpreted as an accumulation of Evans blue in large heterolysosomes. The same experiments were applied to three day magnesium deficient rats. Differences observed from control kidney studies were: increased total kidney Evans blue at 3 hours after injection, increased heavy mitochondrial Evans blue at 3 hours, increased supernatant Evans blue at 3 hours, elevated sertim Evans blue, and increased urine Evans blue at 6 hours after injection. Possible explanations of these differences are discussed in the thesis. Master of Science Pages 42 and 49 are upside-down 2016-04-21T15:35:21Z 2016-04-21T15:35:21Z 1973 Thesis Text http://hdl.handle.net/10919/70557 en OCLC# 33879502 In Copyright http://rightsstatements.org/vocab/InC/1.0/ viii, 88 leaves application/pdf application/pdf Virginia Polytechnic Institute and State University
collection NDLTD
language en
format Others
sources NDLTD
topic LD5655.V855 1973.S43
spellingShingle LD5655.V855 1973.S43
Seignious, George Williams
The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets
description The normal behavior of Evans blue in the vacuolar apparatus of rat kidney and the changes in this normal behavior imposed by a magnesium deficiency were investigated. Since Evans blue binds tightly to serum albumin, and since Evans blue-albumin complexes are reabsorbed from the glomerular filtrate by pinocytosis by the cells of the renal proximal tubules, Evans blue served as a suitable marker of the vacuolar apparatus for these studies. Rats were injected intravenously with Evans blue and sacrificed at either 1/2, 1, 3, or 12 hours post-injection. Kidneys were removed and homogenized. By fractional centrifugations at the different time periods post-injection, the net uptake of Evans blue by the total kidney and each subcellular fraction was observed. The amount of Evans blue taken up by the total kidney was shown to increase with time. Also a gradual increase in the amount of Evans blue in the nuclear fraction with time was seen. This was interpreted as an accumulation of Evans blue in large heterolysosomes. The same experiments were applied to three day magnesium deficient rats. Differences observed from control kidney studies were: increased total kidney Evans blue at 3 hours after injection, increased heavy mitochondrial Evans blue at 3 hours, increased supernatant Evans blue at 3 hours, elevated sertim Evans blue, and increased urine Evans blue at 6 hours after injection. Possible explanations of these differences are discussed in the thesis. === Master of Science === Pages 42 and 49 are upside-down
author2 Biochemistry and Nutrition
author_facet Biochemistry and Nutrition
Seignious, George Williams
author Seignious, George Williams
author_sort Seignious, George Williams
title The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets
title_short The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets
title_full The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets
title_fullStr The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets
title_full_unstemmed The accumulation and distribution of Evans blue in the kidney of rats fed normal or low magnesium diets
title_sort accumulation and distribution of evans blue in the kidney of rats fed normal or low magnesium diets
publisher Virginia Polytechnic Institute and State University
publishDate 2016
url http://hdl.handle.net/10919/70557
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