Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS

Peanut skin, a low-value agriculture waste product, has drawn lots of research interest in recent years, due to its high content of A-type proanthocyanidins. A-type proanthocyanidins have been believed to contribute to cranberries' anti-UTI (urinary tract infection) effect. In this study, we co...

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Main Author: Ye, LiYun
Other Authors: Food Science and Technology
Format: Others
Published: Virginia Tech 2016
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Online Access:http://hdl.handle.net/10919/72283
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spelling ndltd-VTETD-oai-vtechworks.lib.vt.edu-10919-722832021-10-16T05:42:31Z Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS Ye, LiYun Food Science and Technology O'Keefe, Sean F. Neilson, Andrew P. Sarnoski, Paul J. Duncan, Susan E. Boyer, Renee R. peanut skins proanthocyanidins MALDI-TOF Peanut skin, a low-value agriculture waste product, has drawn lots of research interest in recent years, due to its high content of A-type proanthocyanidins. A-type proanthocyanidins have been believed to contribute to cranberries' anti-UTI (urinary tract infection) effect. In this study, we compared the A-type proanthocyanidins in cranberry and peanut skin crude extracts using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Many similarities were found in the proanthocyanidin composition of cranberries and peanut skins. New oligomeric and polymeric proanthocyanidins in peanut skins, including heteroproanthocyanidins and proanthocyanidins with sugar moieties or galloyl esters, were tentatively identified. Solid phase extraction (SPE) and HPLC fractionation largely improved MALDI-TOF's ability to detect proanthocyanidins with high degrees of polymerization (DP). By analyzing the identified compounds in each fraction, we were also able to find some interesting elution pattern of the proanthocyanidins on the SPE cartridges and on the HPLC column. For example, the elution order on both the SPE cartridges and the diol phase column generally followed the DP. A-type proanthocyanidins tended to elute earlier than the B-type. Prodelphinidins retained much longer than other proanthocyanidins with the same DP. These findings may help researcher to identify future research directions and develop new separation methods to facilitate the identification of bioactive components in proanthocyanidin-rich plant extracts. Ph. D. 2016-08-21T06:00:13Z 2016-08-21T06:00:13Z 2015-02-27 Dissertation vt_gsexam:4584 http://hdl.handle.net/10919/72283 In Copyright http://rightsstatements.org/vocab/InC/1.0/ ETD application/pdf Virginia Tech
collection NDLTD
format Others
sources NDLTD
topic peanut skins
proanthocyanidins
MALDI-TOF
spellingShingle peanut skins
proanthocyanidins
MALDI-TOF
Ye, LiYun
Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS
description Peanut skin, a low-value agriculture waste product, has drawn lots of research interest in recent years, due to its high content of A-type proanthocyanidins. A-type proanthocyanidins have been believed to contribute to cranberries' anti-UTI (urinary tract infection) effect. In this study, we compared the A-type proanthocyanidins in cranberry and peanut skin crude extracts using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Many similarities were found in the proanthocyanidin composition of cranberries and peanut skins. New oligomeric and polymeric proanthocyanidins in peanut skins, including heteroproanthocyanidins and proanthocyanidins with sugar moieties or galloyl esters, were tentatively identified. Solid phase extraction (SPE) and HPLC fractionation largely improved MALDI-TOF's ability to detect proanthocyanidins with high degrees of polymerization (DP). By analyzing the identified compounds in each fraction, we were also able to find some interesting elution pattern of the proanthocyanidins on the SPE cartridges and on the HPLC column. For example, the elution order on both the SPE cartridges and the diol phase column generally followed the DP. A-type proanthocyanidins tended to elute earlier than the B-type. Prodelphinidins retained much longer than other proanthocyanidins with the same DP. These findings may help researcher to identify future research directions and develop new separation methods to facilitate the identification of bioactive components in proanthocyanidin-rich plant extracts. === Ph. D.
author2 Food Science and Technology
author_facet Food Science and Technology
Ye, LiYun
author Ye, LiYun
author_sort Ye, LiYun
title Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS
title_short Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS
title_full Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS
title_fullStr Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS
title_full_unstemmed Characterization of A-type Proanthocyanidins in Peanut Skins Using MALDI-TOF MS
title_sort characterization of a-type proanthocyanidins in peanut skins using maldi-tof ms
publisher Virginia Tech
publishDate 2016
url http://hdl.handle.net/10919/72283
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