Microsatellite instability in the evolution of cervical neoplasm.

• Other Authors: Poon, Kin-yan.
• Format: Others
• Language: English; Chinese
• Published: 2001
• Subjects: Uterine Cervical Neoplasms > etiology; Microsatellite Repeats
• Online Access: http://library.cuhk.edu.hk/record=b5890890; http://repository.lib.cuhk.edu.hk/en/item/cuhk-323621

Poon Kin-yan. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. === Includes bibliographical references (leaves 119-147). === Abstracts in English and Chinese. === ACKNOWLEDGMENT --- p.i === ABSTRACT --- p.iii === ABBREVIATIONS --- p.viii === TABLE OF CONTENTS --- p.x === Chapter CHAPTER I --- INTRODUCTION --- p.1 === Chapter 1.1 --- Cervical Intraepithelial Neoplasia (CIN) and Cervical Cancer --- p.1 === Chapter 1.1.1 --- Epidemiology --- p.3 === Chapter 1.1.1.1 --- Descriptive Epidemiology --- p.4 === Chapter 1.1.1.2 --- Risk Factors --- p.7 === Chapter 1.1.2 --- Pathology --- p.22 === Chapter 1.1.2.1 --- Macroscopic Appearance --- p.22 === Chapter 1.1.2.2 --- Symptoms and Diagnosis --- p.23 === Chapter 1.1.2.3 --- Staging Classification --- p.25 === Chapter 1.1.2.4 --- Histopathology --- p.29 === Chapter 1.2 --- Microsatellite Instability (MSI) --- p.35 === Chapter 1.2.1 --- Microsatellite --- p.35 === Chapter 1.2.2 --- Mismatch Repair --- p.37 === Chapter 1.2.3 --- Microsatellite Instability (MSI) --- p.38 === Chapter 1.2.4 --- MSI in Various Cancers --- p.42 === Chapter 1.2.5 --- The Role of MSI in Carcinogenesis --- p.49 === Chapter 1.2.6 --- MSI as a Diagnostic / Prognostic Tool --- p.50 === Chapter CHAPTER II --- AIMS OF THE STUDY --- p.53 === Chapter CHAPTER III --- MATERIALS AND METHODS --- p.56 === Chapter 3.1 --- Materials --- p.56 === Chapter 3.1.1 --- Patients and Specimens --- p.56 === Chapter 3.1.2 --- Microsatellite Markers --- p.57 === Chapter 3.2 --- Methods --- p.59 === Chapter 3.2.1 --- Preparation of OCT-embedded Specimen Sections --- p.59 === Chapter 3.2.2 --- Microdissection of Epithelial Cells and Neoplastic Cells from Specimen Sections --- p.60 === Chapter 3.2.3 --- DNA Extraction --- p.60 === Chapter 3.2.3.1 --- Normal Blood --- p.61 === Chapter 3.2.3.2 --- Dissected Cells --- p.62 === Chapter 3.2.4 --- DNA Amplification --- p.64 === Chapter 3.2.4.1 --- End-labeling of Primers --- p.64 === Chapter 3.2.4.2 --- Polymerase Chain Reaction --- p.65 === Chapter 3.2.5 --- Denaturing Polyacrylamide Gel Electrophoresis --- p.66 === Chapter 3.2.6 --- Autoradiography --- p.67 === Chapter 3.2.7 --- Determination of MSI --- p.67 === Chapter 3.2.8 --- HPV Detection --- p.68 === Chapter 3.2.9 --- Statistical Analysis --- p.69 === Chapter CHAPTER IV --- RESULTS --- p.70 === Chapter 4.1 --- Incidence of MSI in Cervix --- p.70 === Chapter 4.1.1 --- Incidence of MSI in Normal Cervix --- p.70 === Chapter 4.1.2 --- Incidence of MSI in CIN --- p.70 === Chapter 4.1.3 --- Incidence of MSI in Cervical Carcinoma --- p.71 === Chapter 4.1.4 --- Correlation of MSI-positive with the Evolution of Cervical Neoplasm --- p.77 === Chapter 4.2 --- Correlation of MSI-positive with Clinicopathological Characteristics in Cervical Carcinoma --- p.77 === Chapter 4.2.1 --- MSI and Age --- p.80 === Chapter 4.2.2 --- MSI and Clinical Stage --- p.80 === Chapter 4.2.3 --- MSI and Histological Grade --- p.80 === Chapter 4.2.4 --- MSI and Clinical Status --- p.81 === Chapter 4.3 --- Comparison between Two Panels of Microsatellite Markers used in MSI Detection --- p.84 === Chapter 4.4 --- Human Papilloma Virus (HPV) Infection in Cervical Neoplasm --- p.89 === Chapter 4.4.1 --- HPV Infection and Typing in CIN and Cervical Carcinoma --- p.89 === Chapter 4.4.2 --- Correlation of MSI-positive with HPV Infection in Cervical Carcinoma --- p.94 === Chapter CHAPTER V --- DISCUSSION --- p.96 === Chapter 5.1 --- MSI Detection --- p.96 === Chapter 5.1.1 --- Techniques in MSI Assays --- p.98 === Chapter 5.1.2 --- Choice of Microsatellite Markers --- p.101 === Chapter 5.1.3 --- Diagnostic Criteria of MSI --- p.105 === Chapter 5.2 --- The Role of MSI in the Carcinogenesis of Cervical Neoplasm --- p.107 === Chapter 5.3 --- The Clinical Significant of MSI in Cervical Carcinoma --- p.111 === Chapter 5.4 --- The Interaction between HPV Infection and MSI in Cervical Carcinoma --- p.113 === Chapter CHAPTER VI --- CONCLUSION --- p.116 === REFERENCES --- p.119