A biochemical and immunological study of horseradish peroxidase

• Main Author: Odendaal, Ruenda
• Other Authors: Swart, P.
• Language: en
• Published: Stellenbosch : University of Stellenbosch 2009
• Subjects: Horseradish peroxidase; Isoelectric focusing; HPLC; Dissertations > Biochemistry; Theses > Biochemistry; Peroxidase > Analysis; Isoensymes
• Online Access: http://hdl.handle.net/10019.1/1587

Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009. === ENGLISH ABSTRACT: This study describes: a) the isolation and purification of horseradish peroxidase isoenzymes from horseradish roots, b) the characterization of various forms and components of the enzyme by cation-exchange and reversed-phase high performance liquid chromatography, c) the preparation of antibodies against horseradish peroxidase isoenzymes, d) immunological studies for the development of an isoenzyme quantification method and e) the formation of an enzyme-melamine conjugate for use in a melamine quantification immunoassay. === AFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die isolering en suiwering van peperwortel-peroksidase-isoënsieme vanuit die peperwortel, b) die karakterisering van verskillende vorme en komponente van dié ensiem deur katioonuitruilings en omgekeerde-fase HPLC c) die voorbereiding van teenliggaampies vir peperwortel-peroksidase-isoënsieme, d) immunologiese studies vir die ontwikkeling van 'n isoënsiem-kwantifiseringsmetode; en e) die vorming van 'n ensiem-melamien-konjugaat vir gebruik in 'n melamienkwantifiseringsmetode.