DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS

PURPOSE: The purpose of this study is to describe the subgingival bacterial biodiversity in untreated chronic periodontitis patients through the use of next generation 16S rRNA molecular analysis, and to determine similarities or differences between deep and shallow pockets within the same patients...

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Main Author: Rodriguez, Rafael
Format: Others
Published: VCU Scholars Compass 2011
Subjects:
Online Access:http://scholarscompass.vcu.edu/etd/2429
http://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=3428&context=etd
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spelling ndltd-vcu.edu-oai-scholarscompass.vcu.edu-etd-34282017-03-17T08:26:11Z DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS Rodriguez, Rafael PURPOSE: The purpose of this study is to describe the subgingival bacterial biodiversity in untreated chronic periodontitis patients through the use of next generation 16S rRNA molecular analysis, and to determine similarities or differences between deep and shallow pockets within the same patients. METHODS: The analysis involved paired subgingival plaque samples from 24 subjects diagnosed with Generalized Moderate to Severe Chronic Periodontitis. One sample was selected from a single site having a probing depth >5 mm (i.e. Deep Site), and the other from a site with a probing depth <3mm (i.e. Shallow Site) within each subject. Bacterial DNA amplification of the V4-V6 region of the 16S rRNA was performed. The amplicons were sequenced via 454 Roche Genome Sequencer FLX System. The identified sequences were evaluated, and then compared to calculated false discovery rates. RESULTS: A total of 119 independent microbial genera were identified within the samples analyzed. Seven genera were identified to be statistically significant (p<0.05) in their association to deep or shallow sites following t-test and boot strap randomization: Actinomyces (p=0.004), Methylobacterium (p=0.028), Veillonella (p=0.028), and Rothia (p=0.038), and Streptococcus (p=0.033) in Shallow sites; while Mycoplasma (p=0.007) and Fusobacterium (p=0.016) were associated with deep sites. However, taking into account the calculated false discovery rates, it is suggested that none of the 119 microbial genera identified in this study were significantly associated with either deep nor shallow sites. CONCLUSION: The microbial genera identified within this study to be associated with deep and shallow sites follows the traditional pattern anticipated from the literature. However, the calculated false discovery rates suggest that these results may have occurred by chance and not due to a true difference. 2011-05-02T07:00:00Z text application/pdf http://scholarscompass.vcu.edu/etd/2429 http://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=3428&amp;context=etd © The Author Theses and Dissertations VCU Scholars Compass periodontitis microbial flora 16S rRNA chronic Dentistry Medicine and Health Sciences
collection NDLTD
format Others
sources NDLTD
topic periodontitis
microbial flora
16S rRNA
chronic
Dentistry
Medicine and Health Sciences
spellingShingle periodontitis
microbial flora
16S rRNA
chronic
Dentistry
Medicine and Health Sciences
Rodriguez, Rafael
DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS
description PURPOSE: The purpose of this study is to describe the subgingival bacterial biodiversity in untreated chronic periodontitis patients through the use of next generation 16S rRNA molecular analysis, and to determine similarities or differences between deep and shallow pockets within the same patients. METHODS: The analysis involved paired subgingival plaque samples from 24 subjects diagnosed with Generalized Moderate to Severe Chronic Periodontitis. One sample was selected from a single site having a probing depth >5 mm (i.e. Deep Site), and the other from a site with a probing depth <3mm (i.e. Shallow Site) within each subject. Bacterial DNA amplification of the V4-V6 region of the 16S rRNA was performed. The amplicons were sequenced via 454 Roche Genome Sequencer FLX System. The identified sequences were evaluated, and then compared to calculated false discovery rates. RESULTS: A total of 119 independent microbial genera were identified within the samples analyzed. Seven genera were identified to be statistically significant (p<0.05) in their association to deep or shallow sites following t-test and boot strap randomization: Actinomyces (p=0.004), Methylobacterium (p=0.028), Veillonella (p=0.028), and Rothia (p=0.038), and Streptococcus (p=0.033) in Shallow sites; while Mycoplasma (p=0.007) and Fusobacterium (p=0.016) were associated with deep sites. However, taking into account the calculated false discovery rates, it is suggested that none of the 119 microbial genera identified in this study were significantly associated with either deep nor shallow sites. CONCLUSION: The microbial genera identified within this study to be associated with deep and shallow sites follows the traditional pattern anticipated from the literature. However, the calculated false discovery rates suggest that these results may have occurred by chance and not due to a true difference.
author Rodriguez, Rafael
author_facet Rodriguez, Rafael
author_sort Rodriguez, Rafael
title DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS
title_short DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS
title_full DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS
title_fullStr DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS
title_full_unstemmed DEFINING THE BACTERIAL FLORA OF PERIODONTAL POCKETS IN CHRONIC PERIODONTITIS PATIENTS
title_sort defining the bacterial flora of periodontal pockets in chronic periodontitis patients
publisher VCU Scholars Compass
publishDate 2011
url http://scholarscompass.vcu.edu/etd/2429
http://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=3428&amp;context=etd
work_keys_str_mv AT rodriguezrafael definingthebacterialfloraofperiodontalpocketsinchronicperiodontitispatients
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