Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning

Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres t...

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Bibliographic Details
Main Authors: Abu Bakar, N.F (Author), Gen, M. (Author), Lenggoro, I.W (Author), Mokhtar, M.N (Author), Naim, M.N (Author), Saallah, S. (Author)
Format: Article
Language:English
Published: Elsevier B.V. 2016
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Summary:Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film. © 2016 Elsevier B.V. All rights reserved.
ISBN:2215017X (ISSN)
ISSN:2215017X (ISSN)
DOI:10.1016/j.btre.2016.03.003