Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway

Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway

Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator use...

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Bibliographic Details
Main Authors: Brinks, D. (Author), Ganapathy, S. (Author), Meng, X. (Author), Post, M. (Author), van Roemburg, L. (Author)
Format: Article
Language:English
Published: American Chemical Society 2023
Subjects:
biosensors
fluorescence microscopy
genetically encoded voltage indicators
microbial rhodopsins
neuroscience
photophysics
protein engineering
proton transfer pathway
Online Access:View Fulltext in Publisher
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Description
Summary:Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a GEVI subclass known for their high voltage sensitivity and fast response kinetics. Here we review the essential aspects of microbial rhodopsin photocycles that are critical to understanding the mechanisms of voltage sensitivity in these proteins and link them to insights from efforts to create faster, brighter and more sensitive microbial rhodopsin-based GEVIs. © 2023 The Authors. Published by American Chemical Society.
ISBN:26942445 (ISSN)
DOI:10.1021/acsphyschemau.3c00003

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