Gene Editing of Hematopoietic Stem Cells: Hopes and Hurdles Toward Clinical Translation

by Samuele Ferrari, Samuele Ferrari, Valentina Vavassori, Valentina Vavassori, Daniele Canarutto, Daniele Canarutto, Daniele Canarutto, Aurelien Jacob, Aurelien Jacob, Maria Carmina Castiello, Maria Carmina Castiello, Attya Omer Javed, Pietro Genovese, Pietro Genovese, Pietro Genovese, Pietro Genovese
Published 2021-03-01

Methodologies and Challenges for CRISPR/Cas9 Mediated Genome Editing of the Mammalian Brain

by Hirofumi Nishizono, Ryohei Yasuda, Tal Laviv
Published 2020-11-01

From Basic Biology to Patient Mutational Spectra of GATA2 Haploinsufficiencies: What Are the Mechanisms, Hurdles, and Prospects of Genome Editing for Treatment

by Cansu Koyunlar, Emma de Pater
Published 2020-11-01

Protein Phosphatase 2A Catalytic Subunit PP2A-1 Enhances Rice Resistance to Sheath Blight Disease

by Qiu Jun Lin, Jin Chu, Vikranth Kumar, De Peng Yuan, Zhi Min Li, Qiong Mei, Yuan Hu Xuan
Published 2021-02-01

Baboon Envelope Pseudotyped “Nanoblades” Carrying Cas9/gRNA Complexes Allow Efficient Genome Editing in Human T, B, and CD34+ Cells and Knock-in of AAV6-Encoded Donor DNA in CD34+...

by Alejandra Gutierrez-Guerrero, Maria Jimena Abrey Recalde, Maria Jimena Abrey Recalde, Philippe E. Mangeot, Caroline Costa, Ornellie Bernadin, Séverine Périan, Floriane Fusil, Gisèle Froment, Adriana Martinez-Turtos, Adrien Krug, Francisco Martin, Karim Benabdellah, Emiliano P. Ricci, Emiliano P. Ricci, Simone Giovannozzi, Simone Giovannozzi, Rik Gijsbers, Eduard Ayuso, François-Loïc Cosset, Els Verhoeyen, Els Verhoeyen
Published 2021-02-01

Global and Local Manipulation of DNA Repair Mechanisms to Alter Site-Specific Gene Editing Outcomes in Hematopoietic Stem Cells

by Elizabeth K. Benitez, Anastasia Lomova Kaufman, Lilibeth Cervantes, Danielle N. Clark, Paul G. Ayoub, Shantha Senadheera, Kyle Osborne, Julie M. Sanchez, Ralph Valentine Crisostomo, Xiaoyan Wang, Nina Reuven, Yosef Shaul, Roger P. Hollis, Zulema Romero, Donald B. Kohn
Published 2020-12-01

Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

by Li Wang, Hilal Betul Kaya, Hilal Betul Kaya, Ning Zhang, Rhitu Rai, Rhitu Rai, Matthew R. Willmann, Sara C. D. Carpenter, Andrew C. Read, Federico Martin, Zhangjun Fei, Jan E. Leach, Gregory B. Martin, Gregory B. Martin, Adam J. Bogdanove
Published 2021-03-01

Non-immunogenic Induced Pluripotent Stem Cells, a Promising Way Forward for Allogenic Transplantations for Neurological Disorders

by Henriette Reventlow Frederiksen, Ulrik Doehn, Pernille Tveden-Nyborg, Kristine K. Freude
Published 2021-01-01

CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family in Nicotiana tabacum

by Sophie Donovan, Yuwei Mao, Douglas J. Orr, Elizabete Carmo-Silva, Alistair J. McCormick
Published 2020-12-01

Genomic Engineering in Human Hematopoietic Stem Cells: Hype or Hope?

by Stefanie Klaver-Flores, Hidde A. Zittersteijn, Kirsten Canté-Barrett, Arjan Lankester, Rob C. Hoeben, Manuel A. F. V. Gonçalves, Karin Pike-Overzet, Frank J. T. Staal
Published 2021-01-01

Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems in Mammalian Cells

by Daniel Allen, Michael Rosenberg, Ayal Hendel
Published 2021-01-01

Breaking Boundaries in the Brain—Advances in Editing Tools for Neurogenetic Disorders

by Bronte A. Coorey, Bronte A. Coorey, Bronte A. Coorey, Wendy A. Gold, Wendy A. Gold, Wendy A. Gold, Wendy A. Gold, Wendy A. Gold
Published 2021-02-01

Mesenchymal Stromal Cells as a Cellular Target in Myeloid Malignancy: Chances and Challenges in the Genome Editing of Stromal Alterations

by Bella Banjanin, Bella Banjanin, Rebekka K. Schneider, Rebekka K. Schneider, Rebekka K. Schneider
Published 2021-01-01

Delivery Platforms for CRISPR/Cas9 Genome Editing of Glial Cells in the Central Nervous System

by Vasco Meneghini, Marco Peviani, Marco Luciani, Giada Zambonini, Angela Gritti
Published 2021-03-01

A Small Key for a Heavy Door: Genetic Therapies for the Treatment of Hemoglobinopathies

by Hidde A. Zittersteijn, Cornelis L. Harteveld, Stefanie Klaver-Flores, Arjan C. Lankester, Rob C. Hoeben, Frank J. T. Staal, Manuel A. F. V. Gonçalves
Published 2021-02-01

β-Hemoglobinopathies: The Test Bench for Genome Editing-Based Therapeutic Strategies

by Gloria Barbarani, Agata Łabedz, Antonella Ellena Ronchi
Published 2020-12-01

Fast-TrACC: A Rapid Method for Delivering and Testing Gene Editing Reagents in Somatic Plant Cells

by Ryan A. Nasti, Ryan A. Nasti, Ryan A. Nasti, Matthew H. Zinselmeier, Matthew H. Zinselmeier, Matthew H. Zinselmeier, Macy Vollbrecht, Macy Vollbrecht, Macy Vollbrecht, Michael F. Maher, Michael F. Maher, Michael F. Maher, Michael F. Maher, Daniel F. Voytas, Daniel F. Voytas, Daniel F. Voytas
Published 2021-01-01

Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility

by Chengwei Zhang, Guiting Kang, Xinxiang Liu, Si Zhao, Shuang Yuan, Lu Li, Yongxing Yang, Feipeng Wang, Xiang Zhang, Jinxiao Yang
Published 2020-12-01

Precision Genome Engineering Through Cytidine Base Editing in Rapeseed (Brassica napus. L)

by Limin Hu, Olalekan Amoo, Qianqian Liu, Shengli Cai, Miaoshan Zhu, Xiaoxiao Shen, Kaidi Yu, Yungu Zhai, Yang Yang, Lei Xu, Chuchuan Fan, Yongming Zhou
Published 2020-11-01

Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants

by Namie Ohtsuki, Keiko Kizawa, Akiko Mori, Ayako Nishizawa-Yokoi, Ayako Nishizawa-Yokoi, Takao Komatsuda, Hitoshi Yoshida, Katsuyuki Hayakawa, Seiichi Toki, Seiichi Toki, Seiichi Toki, Hiroaki Saika
Published 2021-01-01