real time polymerase chain reaction Article 59 controlled study 52 human 52 genetics 46 Humans 45 gene expression 43 gene expression regulation 36 unclassified drug 34 metabolism 33 upregulation 32 male 30 adult 28 protein expression 28 down regulation 26 female 25 Male 24 Female 23 nonhuman 23 Gene Expression Regulation, Neoplastic 21 Western blotting 21 human cell 21 pathology 21 cell proliferation 20 human tissue 20 middle aged 20 Middle Aged 19 major clinical study 17 microRNA 17 apoptosis 16