| Summary: | Surface-enhanced Raman spectroscopy (SERS) is widely used as a detection method in scientific research fields. However, the method for creating SERS substrates often requires expensive equipment and involves a complex process. Additionally, preserving and effectively utilizing SERS substrates in the long term poses a challenging problem. In order to address these issues, we propose a new method for creating SERS substrates on various types of paper using a combination of a ballpoint pen and 3D printing. This method ensures a high enhancement factor and maximizes the utilization of the substrate. We achieved an enhancement factor of up to 8.2 × 10<sup>8</sup> for detecting R6G molecules, with a relative standard deviation of 11.13% for the Raman peak at 612 cm<sup>−1</sup> of R6G, demonstrating excellent SERS sensitivity and spectral reproducibility. Furthermore, we successfully detected thiram at a concentration as low as 10<sup>−8</sup>, which is lower than both the Chinese national standard and European standard.
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