The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing
The plant viral family Luteoviridae is divided into three genera: Luteovirus, Polerovirus and Enamovirus. Without assistance from another virus, members of the family are confined to the cells of the host plant’s vascular system. The first open reading frame (ORF) of poleroviruses and enamoviruses e...
| 出版年: | Viruses |
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| 主要な著者: | , , , , , , , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
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MDPI AG
2017-10-01
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| オンライン･アクセス: | https://www.mdpi.com/1999-4915/9/10/294 |
| _version_ | 1856934587403337728 |
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| author | Adriana F. Fusaro Deborah A. Barton Kenlee Nakasugi Craig Jackson Melanie L. Kalischuk Lawrence M. Kawchuk Maite F. S. Vaslin Regis L. Correa Peter M. Waterhouse |
| author_facet | Adriana F. Fusaro Deborah A. Barton Kenlee Nakasugi Craig Jackson Melanie L. Kalischuk Lawrence M. Kawchuk Maite F. S. Vaslin Regis L. Correa Peter M. Waterhouse |
| author_sort | Adriana F. Fusaro |
| collection | DOAJ |
| container_title | Viruses |
| description | The plant viral family Luteoviridae is divided into three genera: Luteovirus, Polerovirus and Enamovirus. Without assistance from another virus, members of the family are confined to the cells of the host plant’s vascular system. The first open reading frame (ORF) of poleroviruses and enamoviruses encodes P0 proteins which act as silencing suppressor proteins (VSRs) against the plant’s viral defense-mediating RNA silencing machinery. Luteoviruses, such as barley yellow dwarf virus-PAV (BYDV-PAV), however, have no P0 to carry out the VSR role, so we investigated whether other proteins or RNAs encoded by BYDV-PAV confer protection against the plant’s silencing machinery. Deep-sequencing of small RNAs from plants infected with BYDV-PAV revealed that the virus is subjected to RNA silencing in the phloem tissues and there was no evidence of protection afforded by a possible decoy effect of the highly abundant subgenomic RNA3. However, analysis of VSR activity among the BYDV-PAV ORFs revealed systemic silencing suppression by the P4 movement protein, and a similar, but weaker, activity by P6. The closely related BYDV-PAS P4, but not the polerovirus potato leafroll virus P4, also displayed systemic VSR activity. Both luteovirus and the polerovirus P4 proteins also showed transient, weak local silencing suppression. This suggests that systemic silencing suppression is the principal mechanism by which the luteoviruses BYDV-PAV and BYDV-PAS minimize the effects of the plant’s anti-viral defense. |
| format | Article |
| id | doaj-art-016cfed4e30f41a69e05ffec2e2a3ca6 |
| institution | Directory of Open Access Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-016cfed4e30f41a69e05ffec2e2a3ca62025-08-19T20:12:15ZengMDPI AGViruses1999-49152017-10-0191029410.3390/v9100294v9100294The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA SilencingAdriana F. Fusaro0Deborah A. Barton1Kenlee Nakasugi2Craig Jackson3Melanie L. Kalischuk4Lawrence M. Kawchuk5Maite F. S. Vaslin6Regis L. Correa7Peter M. Waterhouse8School of Life and Environmental Sciences, University of Sydney, Sydney, NSW 2006, AustraliaSchool of Life and Environmental Sciences, University of Sydney, Sydney, NSW 2006, AustraliaSchool of Life and Environmental Sciences, University of Sydney, Sydney, NSW 2006, AustraliaSchool of Life and Environmental Sciences, University of Sydney, Sydney, NSW 2006, AustraliaSchool of Life and Environmental Sciences, University of Sydney, Sydney, NSW 2006, AustraliaSchool of Life and Environmental Sciences, University of Sydney, Sydney, NSW 2006, AustraliaDepartment of Virology (M.F.S.V.), Department of Genetics (R.L.C.) and Institute of Medical Biochemistry (A.F.F.), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-590, BrazilPlant Industry Division, CSIRO, P.O. Box 1600, Canberra, ACT 2601, AustraliaSchool of Life and Environmental Sciences, University of Sydney, Sydney, NSW 2006, AustraliaThe plant viral family Luteoviridae is divided into three genera: Luteovirus, Polerovirus and Enamovirus. Without assistance from another virus, members of the family are confined to the cells of the host plant’s vascular system. The first open reading frame (ORF) of poleroviruses and enamoviruses encodes P0 proteins which act as silencing suppressor proteins (VSRs) against the plant’s viral defense-mediating RNA silencing machinery. Luteoviruses, such as barley yellow dwarf virus-PAV (BYDV-PAV), however, have no P0 to carry out the VSR role, so we investigated whether other proteins or RNAs encoded by BYDV-PAV confer protection against the plant’s silencing machinery. Deep-sequencing of small RNAs from plants infected with BYDV-PAV revealed that the virus is subjected to RNA silencing in the phloem tissues and there was no evidence of protection afforded by a possible decoy effect of the highly abundant subgenomic RNA3. However, analysis of VSR activity among the BYDV-PAV ORFs revealed systemic silencing suppression by the P4 movement protein, and a similar, but weaker, activity by P6. The closely related BYDV-PAS P4, but not the polerovirus potato leafroll virus P4, also displayed systemic VSR activity. Both luteovirus and the polerovirus P4 proteins also showed transient, weak local silencing suppression. This suggests that systemic silencing suppression is the principal mechanism by which the luteoviruses BYDV-PAV and BYDV-PAS minimize the effects of the plant’s anti-viral defense.https://www.mdpi.com/1999-4915/9/10/294LuteoviridaeLuteovirusPolerovirusRNA silencing suppression |
| spellingShingle | Adriana F. Fusaro Deborah A. Barton Kenlee Nakasugi Craig Jackson Melanie L. Kalischuk Lawrence M. Kawchuk Maite F. S. Vaslin Regis L. Correa Peter M. Waterhouse The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing Luteoviridae Luteovirus Polerovirus RNA silencing suppression |
| title | The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing |
| title_full | The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing |
| title_fullStr | The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing |
| title_full_unstemmed | The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing |
| title_short | The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing |
| title_sort | luteovirus p4 movement protein is a suppressor of systemic rna silencing |
| topic | Luteoviridae Luteovirus Polerovirus RNA silencing suppression |
| url | https://www.mdpi.com/1999-4915/9/10/294 |
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