Single-cell heterogeneity and dynamic evolution of Ph-like acute lymphoblastic leukemia patient with novel TPR-PDGFRB fusion gene

• 出版年: Experimental Hematology & Oncology
• 主要な著者: Xuehong Zhang, Zhijie Hou, Dan Huang, Furong Wang, Beibei Gao, Chengtao Zhang, Dong Zhou, Jiacheng Lou, Haina Wang, Yuan Gao, Zhijie Kang, Ying Lu, Quentin Liu, Jinsong Yan
• フォーマット: 論文
• 言語: 英語
• 出版事項: BMC 2023-02-01
• 主題: Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL); Fusion gene; Single-cell RNA sequencing (scRNA-seq); Cellular heterogeneity; Immunotherapy
• オンライン･アクセス: https://doi.org/10.1186/s40164-023-00380-8

Abstract Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL) is a refractory and recurrent subtype of B-cell ALL enriched with kinase-activating rearrangements. Incomplete understanding of the heterogeneity within the tumor cells presents a major challenge for the diagnosis and therapy of Ph-like ALL. Here, we exhibited a comprehensive cell atlas of one Ph-like ALL patient with a novel TPR-PDGFRB fusion gene at diagnosis and relapse by using single-cell RNA sequencing (scRNA-seq). Twelve heterogeneous B-cell clusters, four with strong MKI67 expression indicating highly proliferating B cells, were identified. A relapse-enriched B-cell subset associated with poor prognosis was discovered, implicating the transcriptomic evolution during disease progression. Integrative single-cell analysis was performed on Ph-like ALL and Ph+ ALL patients, and revealed Ph-like specific B-cell subpopulations and shared malignant B cells characterized by the ectopic expression of the inhibitory receptor CLEC2D. Collectively, scRNA-seq of Ph-like ALL with a novel TPR-PDGFRB fusion gene provides valuable insights into the underlying heterogeneity associated with disease progression and offers useful information for the development of immunotherapeutic techniques in the future.