Biogenic Selenium Nanoparticles: A Fine Characterization to Unveil Their Thermodynamic Stability

• Published in: Nanomaterials
• Main Authors: Elena Piacenza, Alessandro Presentato, Francesco Ferrante, Giuseppe Cavallaro, Rosa Alduina, Delia F. Chillura Martino
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Subjects: biogenic selenium nanoparticles; thermodynamic stability; selenium nanorods; <i>Micrococcus</i>; FTIR spectroscopy; DFT calculations
• Online Access: https://www.mdpi.com/2079-4991/11/5/1195

Among the plethora of available metal(loid) nanomaterials (NMs), those containing selenium are interesting from an applicative perspective, due to their high biocompatibility. Microorganisms capable of coping with toxic Se-oxyanions generate mostly Se nanoparticles (SeNPs), representing an ideal and green alternative over the chemogenic synthesis to obtain thermodynamically stable NMs. However, their structural characterization, in terms of biomolecules and interactions stabilizing the biogenic colloidal solution, is still a black hole that impairs the exploitation of biogenic SeNP full potential. Here, spherical and thermodynamically stable SeNPs were produced by a metal(loid) tolerant <i>Micrococcus</i> sp. Structural characterization obtained by Scanning Electron Microscopy (SEM) revealed that these SeNPs were surrounded by an organic material that contributed the most to their electrosteric stabilization, as indicated by Zeta (ζ) potential measurements. Proteins were strongly adsorbed on the SeNP surface, while lipids, polysaccharides, and nucleic acids more loosely interacted with SeNMs as highlighted by Fourier Transform Infrared Spectroscopy (FTIR) and overall supported by multivariate statistical analysis. Nevertheless, all these contributors were fundamental to maintain SeNPs stable, as, upon washing, the NM-containing extract showed the arising of aggregated SeNPs alongside Se nanorods (SeNRs). Besides, Density Functional Theory (DFT) calculation unveiled how thiol-containing molecules appeared to play a role in SeO₃²⁻ bioreduction, stress oxidative response, and SeNP stabilization.