Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

• 出版年: STAR Protocols
• 主要な著者: Clémence Grosjean, Julie Quessada, Mathis Nozais, Marie Loosveld, Dominique Payet-Bornet, Cyrille Mionnet
• フォーマット: 論文
• 言語: 英語
• 出版事項: Elsevier 2021-12-01
• 主題: Cell Biology; Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Cell-based Assays; Immunology
• オンライン･アクセス: http://www.sciencedirect.com/science/article/pii/S2666166721006675
• ISSN: 2666-1667

Summary: Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation.For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021).