Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties
Hemp seeds are rich in metabolites such as protein, lipids and flavonoids, which are beneficial to health and can be used as a nutritional supplement. Few studies have focused on the metabolites of different hemp seed varieties. In the current study, using widely targeted metabolomics based on UHPLC...
| 發表在: | Frontiers in Plant Science |
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| Main Authors: | , , , , , , , , |
| 格式: | Article |
| 語言: | 英语 |
| 出版: |
Frontiers Media S.A.
2022-06-01
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| 主題: | |
| 在線閱讀: | https://www.frontiersin.org/articles/10.3389/fpls.2022.904163/full |
| _version_ | 1857066099936329728 |
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| author | Kang Ning Cong Hou Xiuye Wei Yuxin Zhou Shuanghua Zhang Yongzhong Chen Haibin Yu Linlin Dong Shilin Chen |
| author_facet | Kang Ning Cong Hou Xiuye Wei Yuxin Zhou Shuanghua Zhang Yongzhong Chen Haibin Yu Linlin Dong Shilin Chen |
| author_sort | Kang Ning |
| collection | DOAJ |
| container_title | Frontiers in Plant Science |
| description | Hemp seeds are rich in metabolites such as protein, lipids and flavonoids, which are beneficial to health and can be used as a nutritional supplement. Few studies have focused on the metabolites of different hemp seed varieties. In the current study, using widely targeted metabolomics based on UHPLC-QQQ-MS/MS, we compared the metabolomes of seeds from seven hemp varieties with different uses. A total of 1,001 metabolites, including 201 flavonoids, 86 alkaloids, and 149 phenolic acids, were identified. Flavonoids, organic acids, alkaloids, lipids, and fatty acids with high nutritional value are important to investigate the differences between hemp accessions. By using weighted gene co-expression network analysis (WGCNA), six modules of closely related metabolites were identified. And, we identified the metabolite characteristics and hub metabolites of each variety. Then, we experimentally determined antioxidant activity of seven varieties and demonstrated that alkaloids, flavonoids, phenolic acids, terpenes, and free fatty acids are responsible for the antioxidant activity of hemp seeds. Our research provides useful information for further investigation of the chemical composition of hemp seeds. |
| format | Article |
| id | doaj-art-13466ea05dac45239efc6a196ca86bba |
| institution | Directory of Open Access Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| spelling | doaj-art-13466ea05dac45239efc6a196ca86bba2025-08-19T19:27:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.904163904163Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant PropertiesKang Ning0Cong Hou1Xiuye Wei2Yuxin Zhou3Shuanghua Zhang4Yongzhong Chen5Haibin Yu6Linlin Dong7Shilin Chen8Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaYunnan Hemp Industrial Investment CO.LTD, Kunming, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaHemp seeds are rich in metabolites such as protein, lipids and flavonoids, which are beneficial to health and can be used as a nutritional supplement. Few studies have focused on the metabolites of different hemp seed varieties. In the current study, using widely targeted metabolomics based on UHPLC-QQQ-MS/MS, we compared the metabolomes of seeds from seven hemp varieties with different uses. A total of 1,001 metabolites, including 201 flavonoids, 86 alkaloids, and 149 phenolic acids, were identified. Flavonoids, organic acids, alkaloids, lipids, and fatty acids with high nutritional value are important to investigate the differences between hemp accessions. By using weighted gene co-expression network analysis (WGCNA), six modules of closely related metabolites were identified. And, we identified the metabolite characteristics and hub metabolites of each variety. Then, we experimentally determined antioxidant activity of seven varieties and demonstrated that alkaloids, flavonoids, phenolic acids, terpenes, and free fatty acids are responsible for the antioxidant activity of hemp seeds. Our research provides useful information for further investigation of the chemical composition of hemp seeds.https://www.frontiersin.org/articles/10.3389/fpls.2022.904163/fullhemp seedWGCNAflavonefatty acidantioxidant activity |
| spellingShingle | Kang Ning Cong Hou Xiuye Wei Yuxin Zhou Shuanghua Zhang Yongzhong Chen Haibin Yu Linlin Dong Shilin Chen Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties hemp seed WGCNA flavone fatty acid antioxidant activity |
| title | Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties |
| title_full | Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties |
| title_fullStr | Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties |
| title_full_unstemmed | Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties |
| title_short | Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties |
| title_sort | metabolomics analysis revealed the characteristic metabolites of hemp seeds varieties and metabolites responsible for antioxidant properties |
| topic | hemp seed WGCNA flavone fatty acid antioxidant activity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.904163/full |
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