Microcolony formation from embryogenic callus-derived protoplasts of oil palm
Embryogenic callus of oil palm induced from young leaves of seedlings DxP was used as initial material for protoplast isolation. Various combinations of cellulase Onozuka RS and macerozyme R-10 were tested. Isolated protoplasts were cultured by various methods in MS medium supplemented with differen...
| Published in: | Songklanakarin Journal of Science and Technology (SJST) |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2005-07-01
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| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/27-4-pdf/01-microcolony-palm.pdf |
| Summary: | Embryogenic callus of oil palm induced from young leaves of seedlings DxP was used as initial material for protoplast isolation. Various combinations of cellulase Onozuka RS and macerozyme R-10 were tested. Isolated protoplasts were cultured by various methods in MS medium supplemented with different phytohormones. The results revealed that 2% cellulase RS in combination with 2% macerozyme R-10 (adjusted osmoticum to 0.4 M by manitol) yielded the highest number of viable protoplasts (1x107 per gram fresh weight). Dicamba at concentration 2 mg/l with 1 mg/l 6-benzyladenin (BA) containing in phytagel semisolidified MS medium promoted the highest division of 2.3-4.0%. First division of the protoplasts was observed at 4 days after culture. Microcolony formation (8-10 cells) was seen after three weeks of culture. Unfortunately, neither callus formation nor plantlet regeneration were obtained. |
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| ISSN: | 0125-3395 |