The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreum
Natural rubber is an indispensable material of strategic importance that has critical applications in industry and the military. However, the development of the natural rubber industry is impeded by the red root rot disease of rubber trees caused by Ganoderma pseudoferreum, which is one of the most...
| Published in: | Journal of Integrative Agriculture |
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| Main Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-08-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311924003484 |
| _version_ | 1848769920918093824 |
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| author | Min Tu Zhongfeng Zhu Xinyang Zhao Haibin Cai Yikun Zhang Yichao Yan Ke Yin Zhimin Sha Yi Zhou Gongyou Chen Lifang Zou |
| author_facet | Min Tu Zhongfeng Zhu Xinyang Zhao Haibin Cai Yikun Zhang Yichao Yan Ke Yin Zhimin Sha Yi Zhou Gongyou Chen Lifang Zou |
| author_sort | Min Tu |
| collection | DOAJ |
| container_title | Journal of Integrative Agriculture |
| description | Natural rubber is an indispensable material of strategic importance that has critical applications in industry and the military. However, the development of the natural rubber industry is impeded by the red root rot disease of rubber trees caused by Ganoderma pseudoferreum, which is one of the most devastating diseases in the rubber tree growing regions in China. To combat this disease, we screened the antifungal activity of 223 candidate bacterial strains against G. pseudoferreum, and found that Bacillus velezensis strain SF305 exhibited significant antifungal activity against G. pseudoferreum. Bacillus velezensis SF305 had a nearly 70% efficacy against the red root rot disease of rubber trees with the therapeutic treatment (Tre), while it exhibited over 90% protection effectiveness with the preventive treatment (Pre). The underlying biocontrol mechanism revealed that B. velezensis SF305 could reduce the disease severity of red root rot by degrading the mycelia of G. pseudoferreum. An antiSMASH analysis revealed that B. velezensis SF305 contains 15 gene clusters related to secondary metabolite synthesis, 13 of which are conserved in species of B. velezensis, but surprisingly, B. velezensis SF305 possesses 2 unique secondary metabolite gene clusters. One is predicted to synthesize locillomycin, and the other is a novel nonribosomal peptides synthetase (NRPS) gene cluster. Genomic analysis showed that B. velezensis SF305 harbors genes involved in motility, chemotaxis, biofilm formation, stress resistance, volatile organic compounds (VOCs) and synthesis of the auxin indole-3-acetic acid (IAA), suggesting its plant growth-promoting rhizobacteria (PGPR) properties. Bacillus velezensis SF305 can promote plant growth and efficiently antagonize some important phytopathogenic fungi and bacteria. This study indicates that B. velezensis SF305 is a versatile plant probiotic bacterium. To the best of our knowledge, this is the first time a B. velezensis strain has been reported as a promising biocontrol agent against the red root rot disease of rubber trees. |
| format | Article |
| id | doaj-art-1b21e1abedf248edbf65609f2c19dbe1 |
| institution | Directory of Open Access Journals |
| issn | 2095-3119 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| spelling | doaj-art-1b21e1abedf248edbf65609f2c19dbe12025-10-02T03:08:52ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192025-08-012483112312610.1016/j.jia.2024.09.027The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreumMin Tu0Zhongfeng Zhu1Xinyang Zhao2Haibin Cai3Yikun Zhang4Yichao Yan5Ke Yin6Zhimin Sha7Yi Zhou8Gongyou Chen9Lifang Zou10National Key Laboratory for Tropical Crop Breeding, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572020, ChinaShanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture, Yangtze University, Jingzhou 434000, ChinaNational Key Laboratory for Tropical Crop Breeding, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572020, ChinaShanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture, Yangtze University, Jingzhou 434000, ChinaShanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China; Correspondence Lifang ZouNatural rubber is an indispensable material of strategic importance that has critical applications in industry and the military. However, the development of the natural rubber industry is impeded by the red root rot disease of rubber trees caused by Ganoderma pseudoferreum, which is one of the most devastating diseases in the rubber tree growing regions in China. To combat this disease, we screened the antifungal activity of 223 candidate bacterial strains against G. pseudoferreum, and found that Bacillus velezensis strain SF305 exhibited significant antifungal activity against G. pseudoferreum. Bacillus velezensis SF305 had a nearly 70% efficacy against the red root rot disease of rubber trees with the therapeutic treatment (Tre), while it exhibited over 90% protection effectiveness with the preventive treatment (Pre). The underlying biocontrol mechanism revealed that B. velezensis SF305 could reduce the disease severity of red root rot by degrading the mycelia of G. pseudoferreum. An antiSMASH analysis revealed that B. velezensis SF305 contains 15 gene clusters related to secondary metabolite synthesis, 13 of which are conserved in species of B. velezensis, but surprisingly, B. velezensis SF305 possesses 2 unique secondary metabolite gene clusters. One is predicted to synthesize locillomycin, and the other is a novel nonribosomal peptides synthetase (NRPS) gene cluster. Genomic analysis showed that B. velezensis SF305 harbors genes involved in motility, chemotaxis, biofilm formation, stress resistance, volatile organic compounds (VOCs) and synthesis of the auxin indole-3-acetic acid (IAA), suggesting its plant growth-promoting rhizobacteria (PGPR) properties. Bacillus velezensis SF305 can promote plant growth and efficiently antagonize some important phytopathogenic fungi and bacteria. This study indicates that B. velezensis SF305 is a versatile plant probiotic bacterium. To the best of our knowledge, this is the first time a B. velezensis strain has been reported as a promising biocontrol agent against the red root rot disease of rubber trees.http://www.sciencedirect.com/science/article/pii/S2095311924003484Bacillus velezensisGanoderma pseudoferreumred root rot diseaserubber treebiocontrolcomparative genomics |
| spellingShingle | Min Tu Zhongfeng Zhu Xinyang Zhao Haibin Cai Yikun Zhang Yichao Yan Ke Yin Zhimin Sha Yi Zhou Gongyou Chen Lifang Zou The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreum Bacillus velezensis Ganoderma pseudoferreum red root rot disease rubber tree biocontrol comparative genomics |
| title | The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreum |
| title_full | The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreum |
| title_fullStr | The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreum |
| title_full_unstemmed | The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreum |
| title_short | The versatile plant probiotic bacterium Bacillus velezensis SF305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of Ganoderma pseudoferreum |
| title_sort | versatile plant probiotic bacterium bacillus velezensis sf305 reduces red root rot disease severity in the rubber tree by degrading the mycelia of ganoderma pseudoferreum |
| topic | Bacillus velezensis Ganoderma pseudoferreum red root rot disease rubber tree biocontrol comparative genomics |
| url | http://www.sciencedirect.com/science/article/pii/S2095311924003484 |
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