Modelling the complex nature of the tumor microenvironment: 3D tumor spheroids as an evolving tool
Abstract Cancer remains a serious burden in society and while the pace in the development of novel and more effective therapeutics is increasing, testing platforms that faithfully mimic the tumor microenvironment are lacking. With a clear shift from animal models to more complex in vitro 3D systems,...
| Published in: | Journal of Biomedical Science |
|---|---|
| Main Authors: | Daniel B. Rodrigues, Rui L. Reis, Rogério P. Pirraco |
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
|
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12929-024-00997-9 |
Similar Items
Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis
by: Shrey Shah, et al.
Published: (2025-05-01)
by: Shrey Shah, et al.
Published: (2025-05-01)
EMT and Tumor Turning Point Analysis in 3D Spheroid Culture of HNSCC and Mesenchymal Stem Cells
by: Sabine Brylka, et al.
Published: (2022-12-01)
by: Sabine Brylka, et al.
Published: (2022-12-01)
Development of a Multicellular 3D Tumor Model to Study Cellular Heterogeneity and Plasticity in NSCLC Tumor Microenvironment
by: Leena Arora, et al.
Published: (2022-06-01)
by: Leena Arora, et al.
Published: (2022-06-01)
In vitro models to study natural killer cell dynamics in the tumor microenvironment
by: Valentina Carannante, et al.
Published: (2023-06-01)
by: Valentina Carannante, et al.
Published: (2023-06-01)
3-Dimensional mesothelioma spheroids provide closer to natural pathophysiological tumor microenvironment for drug response studies
by: Huaikai Shi, et al.
Published: (2022-08-01)
by: Huaikai Shi, et al.
Published: (2022-08-01)
Doxorubicin-sensitive and -resistant colorectal cancer spheroid models: assessing tumor microenvironment features for therapeutic modulation
by: Ruben Valente, et al.
Published: (2023-12-01)
by: Ruben Valente, et al.
Published: (2023-12-01)
Characterization of 3D heterocellular spheroids of pancreatic ductal adenocarcinoma for the study of cell interactions in the tumor immune microenvironment
by: Giulio Giustarini, et al.
Published: (2023-07-01)
by: Giulio Giustarini, et al.
Published: (2023-07-01)
From spheroids to organoids: next-generation models for CAR-T cell therapy research in solid tumors
by: Mégane Jassin, et al.
Published: (2025-07-01)
by: Mégane Jassin, et al.
Published: (2025-07-01)
3D Bioprinting: An Important Tool for Tumor Microenvironment Research
by: Li Y, et al.
Published: (2023-12-01)
by: Li Y, et al.
Published: (2023-12-01)
PAX8 modulates the tumor microenvironment of high grade serous ovarian cancer through changes in the secretome
by: Amrita Salvi, et al.
Published: (2023-02-01)
by: Amrita Salvi, et al.
Published: (2023-02-01)
Efficiency of GrowDex<sup>®</sup> nanofibrillar cellulosic hydrogel when generating homotypic and heterotypic 3D tumor spheroids
by: Perumalsamy Balaji, et al.
Published: (2022-08-01)
by: Perumalsamy Balaji, et al.
Published: (2022-08-01)
Capturing the Heterogeneity of the PDAC Tumor Microenvironment: Novel Triple Co-Culture Spheroids for Drug Screening and Angiogenic Evaluation
by: Ruben Verloy, et al.
Published: (2025-03-01)
by: Ruben Verloy, et al.
Published: (2025-03-01)
Characterization of 3D NSCLC Cell Cultures with Fibroblasts or Macrophages for Tumor Microenvironment Studies and Chemotherapy Screening
by: Anali del Milagro Bernabe Garnique, et al.
Published: (2023-12-01)
by: Anali del Milagro Bernabe Garnique, et al.
Published: (2023-12-01)
A patient-derived HCC spheroid system to model the tumor microenvironment and treatment response
by: Emilie Crouchet, et al.
Published: (2025-02-01)
by: Emilie Crouchet, et al.
Published: (2025-02-01)
Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research
by: Bárbara Pinto, et al.
Published: (2020-12-01)
by: Bárbara Pinto, et al.
Published: (2020-12-01)
Tumor Microenvironment
by: Borros Arneth
Published: (2019-12-01)
by: Borros Arneth
Published: (2019-12-01)
Ultrasound-Based Scaffold-Free Core-Shell Multicellular Tumor Spheroid Formation
by: Karl Olofsson, et al.
Published: (2021-03-01)
by: Karl Olofsson, et al.
Published: (2021-03-01)
A Macrophages-Enriched Head and Neck Tumor Spheroid Model to Study Foslip® Behavior in Tumor Microenvironment
by: Francois A, et al.
Published: (2023-11-01)
by: Francois A, et al.
Published: (2023-11-01)
TSG6 affects anti cancer drug resistance and angiogenesis in 3D spheroid model of canine mammary gland tumor cells
by: Ga-Hyun Lim, et al.
Published: (2025-08-01)
by: Ga-Hyun Lim, et al.
Published: (2025-08-01)
Natural medicines target tumor vascular microenvironment to inhibit tumor
by: Yirui Lu, et al.
Published: (2025-11-01)
by: Yirui Lu, et al.
Published: (2025-11-01)
Development of tetraculture spheroids as a versatile 3D model for personalized breast cancer research
by: Oliwia Piwocka, et al.
Published: (2025-07-01)
by: Oliwia Piwocka, et al.
Published: (2025-07-01)
Human Patient-Derived Brain Tumor Models to Recapitulate Ependymoma Tumor Vasculature
by: Min D. Tang-Schomer, et al.
Published: (2023-07-01)
by: Min D. Tang-Schomer, et al.
Published: (2023-07-01)
High-throughput method to analyze the cytotoxicity of CAR-T Cells in a 3D tumor spheroid model using image cytometry
by: David Zurowski, et al.
Published: (2023-04-01)
by: David Zurowski, et al.
Published: (2023-04-01)
Monocyte Infiltration and Differentiation in 3D Multicellular Spheroid Cancer Models
by: Natasha Helleberg Madsen, et al.
Published: (2021-07-01)
by: Natasha Helleberg Madsen, et al.
Published: (2021-07-01)
Tumor spheroid-based and microtumor-based vascularized models for replicating the vascularized tumor microenvironment
by: Jiyoung Song, et al.
Published: (2023-03-01)
by: Jiyoung Song, et al.
Published: (2023-03-01)
Hyaluronic Acid Prevents Fusion of Brain Tumor-Derived Spheroids and Selectively Alters Their Gene Expression Profile
by: Irina Arutyunyan, et al.
Published: (2024-04-01)
by: Irina Arutyunyan, et al.
Published: (2024-04-01)
A promising breakthrough in pancreatic cancer research: The potential of spheroids as 3D models
by: Nazanin Jamshidi, et al.
Published: (2024-05-01)
by: Nazanin Jamshidi, et al.
Published: (2024-05-01)
Applications of Organoids and Spheroids in Anaplastic and Papillary Thyroid Cancer Research: A Comprehensive Review
by: Deepak Gulwani, et al.
Published: (2025-08-01)
by: Deepak Gulwani, et al.
Published: (2025-08-01)
3D biomimetic tumor microenvironment of HCC to visualize the intercellular crosstalk between hepatocytes, hepatic stellate cells, and cancer cells
by: Yaolin Liu, et al.
Published: (2023-01-01)
by: Yaolin Liu, et al.
Published: (2023-01-01)
In Vitro Veritas: From 2D Cultures to Organ-on-a-Chip Models to Study Immunogenic Cell Death in the Tumor Microenvironment
by: Dmitri V. Krysko, et al.
Published: (2022-11-01)
by: Dmitri V. Krysko, et al.
Published: (2022-11-01)
Redefine tumor spheroids heterogeneity via PCA-coupled biophysical characterization
by: Domenico Andrea Cristaldi, et al.
Published: (2025-10-01)
by: Domenico Andrea Cristaldi, et al.
Published: (2025-10-01)
Advances in cancer modeling: fluidic systems for increasing representativeness of large 3D multicellular spheroids
by: Filippo Piccinini, et al.
Published: (2018-12-01)
by: Filippo Piccinini, et al.
Published: (2018-12-01)
Penetration of Nanobody-Dextran Polymer Conjugates through Tumor Spheroids
by: Peter Bitsch, et al.
Published: (2023-09-01)
by: Peter Bitsch, et al.
Published: (2023-09-01)
Structure-to-Efficacy Relationship of HPMA-Based Nanomedicines: The Tumor Spheroid Penetration Study
by: Júlia Kudláčová, et al.
Published: (2020-12-01)
by: Júlia Kudláčová, et al.
Published: (2020-12-01)
Leveraging and manufacturing in vitro multicellular spheroid-based tumor cell model as a preclinical tool for translating dysregulated tumor metabolism into clinical targets and biomarkers
by: Tong Wang, et al.
Published: (2020-07-01)
by: Tong Wang, et al.
Published: (2020-07-01)
Spatial Metabolomics Profiling Reveals Curcumin Induces Metabolic Reprogramming in Three-Dimensional Tumor Spheroids
by: Zihan Zhu, et al.
Published: (2024-09-01)
by: Zihan Zhu, et al.
Published: (2024-09-01)
Tumor microenvironment reprogramming by nanomedicine to enhance the effect of tumor immunotherapy
by: Yu Huang, et al.
Published: (2024-04-01)
by: Yu Huang, et al.
Published: (2024-04-01)
Assessing Polysaccharides/Aloe Vera–Based Hydrogels for Tumor Spheroid Formation
by: Petruța Preda, et al.
Published: (2023-01-01)
by: Petruța Preda, et al.
Published: (2023-01-01)
The Tumor Microenvironment: Signal Transduction
by: Xianhong Zhang, et al.
Published: (2024-04-01)
by: Xianhong Zhang, et al.
Published: (2024-04-01)
Atypical Chemokine Receptor CCRL2 Shapes Tumor Spheroid Structure and Immune Signaling in Melanoma
by: Diana Al Delbany, et al.
Published: (2025-08-01)
by: Diana Al Delbany, et al.
Published: (2025-08-01)
Similar Items
-
Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis
by: Shrey Shah, et al.
Published: (2025-05-01) -
EMT and Tumor Turning Point Analysis in 3D Spheroid Culture of HNSCC and Mesenchymal Stem Cells
by: Sabine Brylka, et al.
Published: (2022-12-01) -
Development of a Multicellular 3D Tumor Model to Study Cellular Heterogeneity and Plasticity in NSCLC Tumor Microenvironment
by: Leena Arora, et al.
Published: (2022-06-01) -
In vitro models to study natural killer cell dynamics in the tumor microenvironment
by: Valentina Carannante, et al.
Published: (2023-06-01) -
3-Dimensional mesothelioma spheroids provide closer to natural pathophysiological tumor microenvironment for drug response studies
by: Huaikai Shi, et al.
Published: (2022-08-01)
