Platelet Hyperactivation Plays a Critical Role in Exacerbating Skin Lesions in Rats with Psoriasis and Blood Stasis Syndrome

• 出版年: Journal of Inflammation Research
• 主要な著者: Yue H, Mo H, Su H, Zeng Z, Liu F, Lei C, Sun Y, Wang T, Pi X, Li L, Wu J, Han L
• フォーマット: 論文
• 言語: 英語
• 出版事項: Dove Medical Press 2025-10-01
• 主題: psoriasis with blood stasis syndrome; platelet activation; Clopidogrel; P2ry12
• オンライン･アクセス: https://www.dovepress.com/platelet-hyperactivation-plays-a-critical-role-in-exacerbating-skin-le-peer-reviewed-fulltext-article-JIR

Hongyu Yue,1 Haoran Mo,1 Haojie Su,1 Zizhong Zeng,2 Fanlu Liu,1 Chenjing Lei,1 Yue Sun,1 Tingyu Wang,1 Xiaorui Pi,1 Li Li,1 Jingjing Wu,1 Ling Han3– 7 1Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, People’s Republic of China; 2Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI, USA; 3Chinese Medicine Guangdong Laboratory, Zhuhai, Guangdong Province, People’s Republic of China; 4State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, People’s Republic of China; 5Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou, Guangdong Province, People’s Republic of China; 6Guangdong-Hong Kong-Macau Joint Laboratory on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, People’s Republic of China; 7Research Team of Bio-Molecular and System Biology of Chinese Medicine, Guangdong Academy of Traditional Chinese Medicine, Guangzhou, Guangdong Province, People’s Republic of ChinaCorrespondence: Ling Han, Email [e-mail]: Psoriasis with blood stasis syndrome (BSS) demonstrates aggravated skin lesions compared to psoriasis alone; however, the underlying mechanism remains unclear. This study aims to elucidate the pathological mechanisms of skin lesion exacerbation in psoriasis from the perspective of platelet activation.Methods: A psoriasis rat model was established by topical application of imiquimod (IMQ), while a psoriasis with BSS model was induced using ice–water baths and epinephrine injection. Skin lesions were assessed via hematoxylin and eosin (H&E) staining. Hemorheology was employed to evaluate BSS characteristics. Western blot (WB) was used to examine CD62P (P-selectin) and platelet-activating factor receptor (PAFR) expression to assess platelet activation. The platelet aggregation inhibitor clopidogrel was administered via oral gavage. The psoriasis area and severity index (PASI) was applied to evaluate clopidogrel’s therapeutic effect, and network pharmacology combined with quantitative reverse transcription PCR (RT–qPCR) was used to clarify its mechanism.Results: HE staining indicated more severe skin lesions in psoriasis with BSS rats than in psoriasis rats (P< 0.01). Hemorheological analysis revealed increased blood viscosity and microvascular proliferation in the psoriasis rats with BSS. WB showed significantly elevated expression of CD62P and PAFR in psoriasis with BSS rats (P< 0.05). Clopidogrel reduced epidermal thickening, as assessed by the PASI score. Network pharmacology and RT–qPCR identified P2RY12 and GPIIb/IIIa as key targets through which clopidogrel ameliorates skin lesions in psoriasis with BSS.Conclusion: Psoriasis with BSS rats exhibit more severe skin lesions than psoriasis rats, associated with enhanced platelet activation. Clopidogrel improved blood stasis and skin inflammation, confirming that platelet activation contributes critically to skin lesion worsening.Keywords: psoriasis with blood stasis syndrome, platelet activation, clopidogrel, P2ry12