Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum

• Published in: 浙江大学学报. 农业与生命科学版
• Main Authors: DAI Fu-ming, XU Tong
• Format: Article
• Language: English
• Published: Zhejiang University Press 2004-07-01
• Subjects: <italic>Arabidopsis thaliana</italic>; <italic>Trichoderma</italic> endochitinase gene; resistance; <italic>Sclerotinia sclerotiorum</italic>
• Online Access: https://www.academax.com/doi/10.3785/1008-9209.2004.04.0426
• ISSN: 1008-9209; 2097-5155

Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Co10) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase (ThEn-42) was amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation. In addition, an enhanced resistance to S. sclerotiorum was observed. The mortality percentage (7.7% to 33.3%) in transgenic plants was significantly lower than in non-transgenic plants (86.7%) 10 days after inoculation with the pathogen.