Method for 96-Well M13 DNA Template Preparations for Large-Scale Sequencing
Efficient preparation of DNA templates is an important step in large-scale DNA sequencing. To ensure high-quality sequence data, we have prepared M13 phage DNA templates using a glass fiber-filtration method. We present the adaptation of this protocol to a 96-well format using commercially available...
| Published in: | BioTechniques |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-06-01
|
| Online Access: | https://www.future-science.com/doi/10.2144/96206st03 |
| Summary: | Efficient preparation of DNA templates is an important step in large-scale DNA sequencing. To ensure high-quality sequence data, we have prepared M13 phage DNA templates using a glass fiber-filtration method. We present the adaptation of this protocol to a 96-well format using commercially available filter plates. Two variations are described: one using polyethylene glycol precipitation and a second where the phage particles are disrupted before filtration, thus eliminating the need for precipitation. Using either of these protocols, 96 templates can be prepared in less than 2 h. Sufficient DNA for 1–2 dye primer sequencing reactions is routinely obtained from 1 mL of culture, and the resulting sequence data are of high quality. |
|---|---|
| ISSN: | 0736-6205 1940-9818 |