Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition Dysfunction
The aim of this study is to determine the molecular differences between the urothelial transcriptomes of the bladder body and trigone. The transcriptomes of the bladder body and trigonal epithelia were analyzed by massive sequencing of total epithelial RNA. The profiles of urothelial and urinal micr...
| Published in: | Biomedicines |
|---|---|
| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/6/1435 |
| _version_ | 1851075822595604480 |
|---|---|
| author | Natalia Zeber-Lubecka Maria Kulecka Katarzyna Załęska-Oracka Michalina Dąbrowska Aneta Bałabas Ewa E. Hennig Magdalena Szymanek-Szwed Michał Mikula Beata Jurkiewicz Jerzy Ostrowski |
| author_facet | Natalia Zeber-Lubecka Maria Kulecka Katarzyna Załęska-Oracka Michalina Dąbrowska Aneta Bałabas Ewa E. Hennig Magdalena Szymanek-Szwed Michał Mikula Beata Jurkiewicz Jerzy Ostrowski |
| author_sort | Natalia Zeber-Lubecka |
| collection | DOAJ |
| container_title | Biomedicines |
| description | The aim of this study is to determine the molecular differences between the urothelial transcriptomes of the bladder body and trigone. The transcriptomes of the bladder body and trigonal epithelia were analyzed by massive sequencing of total epithelial RNA. The profiles of urothelial and urinal microbiomes were assessed by amplicon sequencing of bacterial 16S rRNA genes in 17 adolescent females with pain and micturition dysfunction and control female subjects. The RNA sequencing identified 10,261 differentially expressed genes (DEGs) in the urothelia of the bladder body and trigone, with the top 1000 DEGs at these locations annotated to 36 and 77 of the Reactome-related pathways in the bladder body and trigone, respectively. These pathways represented 11 categories enriched in the bladder body urothelium, including <i>extracellular matrix organization</i>, <i>the neuronal system</i>, and 15 categories enriched in the trigonal epithelium, including <i>RHO GTPase effectors</i>, <i>cornified envelope formation</i>, and <i>neutrophil degranulation</i>. Five bacterial taxa in urine differed significantly in patients and healthy adolescent controls. The evaluation of their transcriptomes indicated that the bladder body and trigonal urothelia were functionally different tissues. The molecular differences between the body and trigonal urothelia responsible for clinical symptoms in adolescents with bladder pain syndrome/interstitial cystitis remain unclear. |
| format | Article |
| id | doaj-art-2efab2d459804ea0968d32eebd1de648 |
| institution | Directory of Open Access Journals |
| issn | 2227-9059 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-2efab2d459804ea0968d32eebd1de6482025-08-19T22:33:39ZengMDPI AGBiomedicines2227-90592022-06-01106143510.3390/biomedicines10061435Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition DysfunctionNatalia Zeber-Lubecka0Maria Kulecka1Katarzyna Załęska-Oracka2Michalina Dąbrowska3Aneta Bałabas4Ewa E. Hennig5Magdalena Szymanek-Szwed6Michał Mikula7Beata Jurkiewicz8Jerzy Ostrowski9Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Roentgena 5, 02-781 Warsaw, PolandDepartment of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Roentgena 5, 02-781 Warsaw, PolandDepartment of Pediatric Surgery, Children’s Hospital, Centre of Postgraduate Medical Education, Marii Konopnickiej 65, 05-092 Dziekanow Lesny, PolandDepartment of Genetics, Maria Sklodowska-Curie National Institute of Oncology, Roentgena 5, 02-781 Warsaw, PolandDepartment of Genetics, Maria Sklodowska-Curie National Institute of Oncology, Roentgena 5, 02-781 Warsaw, PolandDepartment of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Roentgena 5, 02-781 Warsaw, PolandDepartment of Pediatric Surgery, Children’s Hospital, Centre of Postgraduate Medical Education, Marii Konopnickiej 65, 05-092 Dziekanow Lesny, PolandDepartment of Genetics, Maria Sklodowska-Curie National Institute of Oncology, Roentgena 5, 02-781 Warsaw, PolandDepartment of Pediatric Surgery, Children’s Hospital, Centre of Postgraduate Medical Education, Marii Konopnickiej 65, 05-092 Dziekanow Lesny, PolandDepartment of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Roentgena 5, 02-781 Warsaw, PolandThe aim of this study is to determine the molecular differences between the urothelial transcriptomes of the bladder body and trigone. The transcriptomes of the bladder body and trigonal epithelia were analyzed by massive sequencing of total epithelial RNA. The profiles of urothelial and urinal microbiomes were assessed by amplicon sequencing of bacterial 16S rRNA genes in 17 adolescent females with pain and micturition dysfunction and control female subjects. The RNA sequencing identified 10,261 differentially expressed genes (DEGs) in the urothelia of the bladder body and trigone, with the top 1000 DEGs at these locations annotated to 36 and 77 of the Reactome-related pathways in the bladder body and trigone, respectively. These pathways represented 11 categories enriched in the bladder body urothelium, including <i>extracellular matrix organization</i>, <i>the neuronal system</i>, and 15 categories enriched in the trigonal epithelium, including <i>RHO GTPase effectors</i>, <i>cornified envelope formation</i>, and <i>neutrophil degranulation</i>. Five bacterial taxa in urine differed significantly in patients and healthy adolescent controls. The evaluation of their transcriptomes indicated that the bladder body and trigonal urothelia were functionally different tissues. The molecular differences between the body and trigonal urothelia responsible for clinical symptoms in adolescents with bladder pain syndrome/interstitial cystitis remain unclear.https://www.mdpi.com/2227-9059/10/6/1435bladder body urotheliummicturition dysfunctionmicrobiometrigonal urothelium |
| spellingShingle | Natalia Zeber-Lubecka Maria Kulecka Katarzyna Załęska-Oracka Michalina Dąbrowska Aneta Bałabas Ewa E. Hennig Magdalena Szymanek-Szwed Michał Mikula Beata Jurkiewicz Jerzy Ostrowski Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition Dysfunction bladder body urothelium micturition dysfunction microbiome trigonal urothelium |
| title | Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition Dysfunction |
| title_full | Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition Dysfunction |
| title_fullStr | Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition Dysfunction |
| title_full_unstemmed | Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition Dysfunction |
| title_short | Gene Expression-Based Functional Differences between the Bladder Body and Trigonal Urothelium in Adolescent Female Patients with Micturition Dysfunction |
| title_sort | gene expression based functional differences between the bladder body and trigonal urothelium in adolescent female patients with micturition dysfunction |
| topic | bladder body urothelium micturition dysfunction microbiome trigonal urothelium |
| url | https://www.mdpi.com/2227-9059/10/6/1435 |
| work_keys_str_mv | AT nataliazeberlubecka geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT mariakulecka geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT katarzynazałeskaoracka geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT michalinadabrowska geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT anetabałabas geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT ewaehennig geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT magdalenaszymanekszwed geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT michałmikula geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT beatajurkiewicz geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction AT jerzyostrowski geneexpressionbasedfunctionaldifferencesbetweenthebladderbodyandtrigonalurotheliuminadolescentfemalepatientswithmicturitiondysfunction |