| Summary: | Post-operative endophthalmitis caused by <i>Enterococcus</i> spp. progresses rapidly and often results in substantial and irreversible vision loss. Therefore, novel alternative treatments that are effective against enterococcal endophthalmitis are required. Bacteriophage therapy has the potential to be an optional therapy for infectious diseases. Therefore, we investigated the therapeutic potential of three newly isolated enterococcal phages, phiEF7H, phiEF14H1, and phiEF19G, in <i>E. faecalis</i>-induced endophthalmitis. These phages could lyse the broad-range <i>E. faecalis</i>, including strains derived from endophthalmitis and vancomycin-resistant <i>E. faecalis</i> in vitro, as determined by the streak test. Morphological and genomic analyses revealed that these phages were classified into the <i>Herelleviridae</i> genus <i>Kochikohdavirus</i>. The whole genomes of these phages contained 143,399, 143,280, and 143,400 bp, respectively. Endophthalmitis was induced in mice by injection of three strains of <i>E. faecalis</i> derived from post-operative endophthalmitis or vancomycin-resistant strains into the vitreous body. The number of viable bacteria and infiltration of neutrophils in the eye were both decreased by intravitreous injection of phiEF7H, phiEF14H1, and phiEF19G 6 h after injection of all <i>E. faecalis</i> strains. Thus, these results suggest that these newly isolated phages may serve as promising candidates for phage therapy against endophthalmitis.
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