| Summary: | [Objective] TIFY gene are involved in the regulation of growth and development in plants. This
experiment involves the identification of the TIFY family members in Luffa cylindrica, selecting the
highly expressed members in fruits, and further cloning and investigating their expression patterns in different
tissues, in order to provide basis for the functional identification of these genes. [Methods] Bioinformatics
methods were used to identify the TIFY gene family members in L. cylindrica. Transcriptome
analysis was performed to screen the highly expressed members in L. cylindrica fruits, followed by cloning
genes using RT-PCR. Tissue expression was analyzed using qRT-PCR. [Results] L. cylindrica genome
contains 17 TIFY genes, which were unevenly distributed across 9 chromosomes, with CDS lengths
ranging from 297 bp to 1 353 bp, classifing into four subfamilies. Transcriptome analysis revealed that
LcTIFY2, LcTIFY7, LcTIFY11, and LcTIFY13 were highly expressed in L. cylindrica fruits. The
CDS sequences of these genes were cloned, comprising 3, 5, 6, and 2 exons, respectively. Tissue expression
analysis indicated that all four genes had the highest expression in the peel of L. cylindrica fruits.
Additionally, LcTIFY7 and LcTIFY13 showed significantly higher expression in reproductive organs,
while LcTIFY11 exhibited significantly higher expression in female flower buds and ovaries. [Conclusion]
The TIFY family members LcTIFY2, LcTIFY7, LcTIFY11 and LcTIFY13 may play roles in reproductive
growth and fruit development in L. cylindrica.
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