Extending Imaging Volume in Soft X‐Ray Tomography
Soft X‐ray tomography offers rapid imaging of whole, single cells with a few tens of nanometers spatial resolution without fixation or labeling. Herein, this technique is limited to specimens about 10 μm thick, such that applications of soft X‐ray tomography of large human cells or multicellular spe...
| 發表在: | Advanced Photonics Research |
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| Main Authors: | , , , , , , |
| 格式: | Article |
| 語言: | 英语 |
| 出版: |
Wiley-VCH
2023-04-01
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| 主題: | |
| 在線閱讀: | https://doi.org/10.1002/adpr.202200142 |
| _version_ | 1851893138656854016 |
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| author | Axel Ekman Jian-Hua Chen Bieke Vanslembrouck Valentina Loconte Carolyn A. Larabell Mark A. Le Gros Venera Weinhardt |
| author_facet | Axel Ekman Jian-Hua Chen Bieke Vanslembrouck Valentina Loconte Carolyn A. Larabell Mark A. Le Gros Venera Weinhardt |
| author_sort | Axel Ekman |
| collection | DOAJ |
| container_title | Advanced Photonics Research |
| description | Soft X‐ray tomography offers rapid imaging of whole, single cells with a few tens of nanometers spatial resolution without fixation or labeling. Herein, this technique is limited to specimens about 10 μm thick, such that applications of soft X‐ray tomography of large human cells or multicellular specimens are not possible. A theoretical and experimental framework for soft X‐ray tomography that enables extension of imaging volumes to 18 μm‐thick specimens is developed. This approach, based on long depth of field and half‐acquisition tomography, is easily applicable to microscopes equipped with a full‐rotation specimen stage. This opens opportunities for imaging large human cells, such as those commonly seen in cancer research, as well as cell‐to‐cell interactions, where two or more cells occupy the same imaging volume. |
| format | Article |
| id | doaj-art-34ab2b85a3cf4a76a5ee7c6e7e7cfd72 |
| institution | Directory of Open Access Journals |
| issn | 2699-9293 |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| spelling | doaj-art-34ab2b85a3cf4a76a5ee7c6e7e7cfd722025-08-19T22:08:50ZengWiley-VCHAdvanced Photonics Research2699-92932023-04-0144n/an/a10.1002/adpr.202200142Extending Imaging Volume in Soft X‐Ray TomographyAxel Ekman0Jian-Hua Chen1Bieke Vanslembrouck2Valentina Loconte3Carolyn A. Larabell4Mark A. Le Gros5Venera Weinhardt6Department of Anatomy University of California San Francisco San Francisco CA 94143 USADepartment of Anatomy University of California San Francisco San Francisco CA 94143 USADepartment of Anatomy University of California San Francisco San Francisco CA 94143 USADepartment of Anatomy University of California San Francisco San Francisco CA 94143 USADepartment of Anatomy University of California San Francisco San Francisco CA 94143 USADepartment of Anatomy University of California San Francisco San Francisco CA 94143 USADepartment of Anatomy University of California San Francisco San Francisco CA 94143 USASoft X‐ray tomography offers rapid imaging of whole, single cells with a few tens of nanometers spatial resolution without fixation or labeling. Herein, this technique is limited to specimens about 10 μm thick, such that applications of soft X‐ray tomography of large human cells or multicellular specimens are not possible. A theoretical and experimental framework for soft X‐ray tomography that enables extension of imaging volumes to 18 μm‐thick specimens is developed. This approach, based on long depth of field and half‐acquisition tomography, is easily applicable to microscopes equipped with a full‐rotation specimen stage. This opens opportunities for imaging large human cells, such as those commonly seen in cancer research, as well as cell‐to‐cell interactions, where two or more cells occupy the same imaging volume.https://doi.org/10.1002/adpr.202200142opticstomographyX-rays |
| spellingShingle | Axel Ekman Jian-Hua Chen Bieke Vanslembrouck Valentina Loconte Carolyn A. Larabell Mark A. Le Gros Venera Weinhardt Extending Imaging Volume in Soft X‐Ray Tomography optics tomography X-rays |
| title | Extending Imaging Volume in Soft X‐Ray Tomography |
| title_full | Extending Imaging Volume in Soft X‐Ray Tomography |
| title_fullStr | Extending Imaging Volume in Soft X‐Ray Tomography |
| title_full_unstemmed | Extending Imaging Volume in Soft X‐Ray Tomography |
| title_short | Extending Imaging Volume in Soft X‐Ray Tomography |
| title_sort | extending imaging volume in soft x ray tomography |
| topic | optics tomography X-rays |
| url | https://doi.org/10.1002/adpr.202200142 |
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