Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus
Blackberry chlorotic ringspot virus (BCRV) was described about 20 years ago and since then there have been several publications of the virus infecting rosaceous hosts including blackberry, raspberry, rose and apple at high rates. Still the effect of the virus on disease development is poorly underst...
| 出版年: | Virus Research |
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| 主要な著者: | , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
Elsevier
2024-12-01
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| 主題: | |
| オンライン･アクセス: | http://www.sciencedirect.com/science/article/pii/S0168170224001539 |
| _version_ | 1849522368523796480 |
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| author | Andrea Sierra-Mejia Dan E.V. Villamor Ioannis E. Tzanetakis |
| author_facet | Andrea Sierra-Mejia Dan E.V. Villamor Ioannis E. Tzanetakis |
| author_sort | Andrea Sierra-Mejia |
| collection | DOAJ |
| container_title | Virus Research |
| description | Blackberry chlorotic ringspot virus (BCRV) was described about 20 years ago and since then there have been several publications of the virus infecting rosaceous hosts including blackberry, raspberry, rose and apple at high rates. Still the effect of the virus on disease development is poorly understood. Aiming to bridge this knowledge gap, we developed a BCRV infectious clone and virus-induced gene silencing vector (VIGS). The infectious clone can induce systemic infection with the transmissibility of the recombinant virus evaluated through mechanical transmission. The VIGS induced silencing using two different inserts, proving the versatility of the construct. The products of this work can be used to study disease development and control as well as functional genomics studies of BCRV hosts. |
| format | Article |
| id | doaj-art-34f5ab643ff147e7a272b9fc902cfb39 |
| institution | Directory of Open Access Journals |
| issn | 1872-7492 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-34f5ab643ff147e7a272b9fc902cfb392025-08-20T02:52:26ZengElsevierVirus Research1872-74922024-12-0135019946010.1016/j.virusres.2024.199460Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virusAndrea Sierra-Mejia0Dan E.V. Villamor1Ioannis E. Tzanetakis2Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System Fayetteville, AR, 72701, USADepartment of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System Fayetteville, AR, 72701, USACorresponding author.; Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System Fayetteville, AR, 72701, USABlackberry chlorotic ringspot virus (BCRV) was described about 20 years ago and since then there have been several publications of the virus infecting rosaceous hosts including blackberry, raspberry, rose and apple at high rates. Still the effect of the virus on disease development is poorly understood. Aiming to bridge this knowledge gap, we developed a BCRV infectious clone and virus-induced gene silencing vector (VIGS). The infectious clone can induce systemic infection with the transmissibility of the recombinant virus evaluated through mechanical transmission. The VIGS induced silencing using two different inserts, proving the versatility of the construct. The products of this work can be used to study disease development and control as well as functional genomics studies of BCRV hosts.http://www.sciencedirect.com/science/article/pii/S0168170224001539RubusIlarvirusTransmissionDetection |
| spellingShingle | Andrea Sierra-Mejia Dan E.V. Villamor Ioannis E. Tzanetakis Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus Rubus Ilarvirus Transmission Detection |
| title | Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus |
| title_full | Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus |
| title_fullStr | Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus |
| title_full_unstemmed | Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus |
| title_short | Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus |
| title_sort | development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus |
| topic | Rubus Ilarvirus Transmission Detection |
| url | http://www.sciencedirect.com/science/article/pii/S0168170224001539 |
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