Isolation and culture of protoplast from leaves of Lactuca sativa

Protoplasts were isolated from leaves of lettuce (Lactuca sativa L.) seedlings after in vitro germination for 25, 30, 40 and 50 days. The leaves were stripped and incubated in various combinations of cellulase and pectinase. Protoplasts were cultured on MS medium containing various kinds and concent...

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Bibliographic Details
Published in:	Songklanakarin Journal of Science and Technology (SJST)
Main Author:	Witool Chaipakdee
Format:	Article
Language:	English
Published:	Prince of Songkla University 2007-07-01
Subjects:	protoplasts lettuce isolation microcolony formation
Online Access:	http://www.sjst.psu.ac.th/journal/29-4_online/0125-3395-29-4-0929-0935.pdf

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