Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts
microRNAs regulate subcellular functions through distinct molecular mechanisms. In this study, we used normal and pathogenic fibroblasts in pelvic fracture urethral distraction defects (PFUDD) patients. PFUDD is a common disease that could severely affect patients’ life quality, yet little is known...
| Published in: | Frontiers in Bioengineering and Biotechnology |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-05-01
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| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00428/full |
| _version_ | 1852817477655855104 |
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| author | Kaile Zhang Kaile Zhang Ranxin Yang Ranxin Yang Jun Chen Jun Chen Er Qi Shukui Zhou Ying Wang Qiang Fu Qiang Fu Rong Chen Rong Chen Xiaolan Fang |
| author_facet | Kaile Zhang Kaile Zhang Ranxin Yang Ranxin Yang Jun Chen Jun Chen Er Qi Shukui Zhou Ying Wang Qiang Fu Qiang Fu Rong Chen Rong Chen Xiaolan Fang |
| author_sort | Kaile Zhang |
| collection | DOAJ |
| container_title | Frontiers in Bioengineering and Biotechnology |
| description | microRNAs regulate subcellular functions through distinct molecular mechanisms. In this study, we used normal and pathogenic fibroblasts in pelvic fracture urethral distraction defects (PFUDD) patients. PFUDD is a common disease that could severely affect patients’ life quality, yet little is known about the molecular mechanism associated with pathogenic fibrosis in PFUDD. Our data showed that let-7i-5p performs a multi-functional role in distinct signaling transduction pathways involved in cell morphology and cell migration in both normal and pathogenic fibroblasts. By analyzing the molecular mechanism associated with its functions, we found that let-7i-5p regulates through its direct target genes involved in collagen metabolism, cell proliferation and differentiation, TGF-beta signaling, DNA repair and ubiquitination, gene silencing and oxygen homeostasis. We conclude that let-7i-5p plays an essential role in regulating cell shape and tissue elasticity, cell migration, cell morphology and cytoskeleton, and could serve as a potential target for clinical treatment of urethral stricture patients. |
| format | Article |
| id | doaj-art-3c0f30899058461bb3cfcecd45fca17e |
| institution | Directory of Open Access Journals |
| issn | 2296-4185 |
| language | English |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| spelling | doaj-art-3c0f30899058461bb3cfcecd45fca17e2025-08-19T20:33:36ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-05-01810.3389/fbioe.2020.00428512558Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral FibroblastsKaile Zhang0Kaile Zhang1Ranxin Yang2Ranxin Yang3Jun Chen4Jun Chen5Er Qi6Shukui Zhou7Ying Wang8Qiang Fu9Qiang Fu10Rong Chen11Rong Chen12Xiaolan FangThe Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Eastern Institute of Urologic Reconstruction, Shanghai, ChinaThe Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Eastern Institute of Urologic Reconstruction, Shanghai, ChinaThe Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Eastern Institute of Urologic Reconstruction, Shanghai, ChinaShanghai Xuhui District Xietu Street Community Health Service Center, Shanghai, ChinaThe Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThe Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThe Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Eastern Institute of Urologic Reconstruction, Shanghai, ChinaThe Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Eastern Institute of Urologic Reconstruction, Shanghai, ChinamicroRNAs regulate subcellular functions through distinct molecular mechanisms. In this study, we used normal and pathogenic fibroblasts in pelvic fracture urethral distraction defects (PFUDD) patients. PFUDD is a common disease that could severely affect patients’ life quality, yet little is known about the molecular mechanism associated with pathogenic fibrosis in PFUDD. Our data showed that let-7i-5p performs a multi-functional role in distinct signaling transduction pathways involved in cell morphology and cell migration in both normal and pathogenic fibroblasts. By analyzing the molecular mechanism associated with its functions, we found that let-7i-5p regulates through its direct target genes involved in collagen metabolism, cell proliferation and differentiation, TGF-beta signaling, DNA repair and ubiquitination, gene silencing and oxygen homeostasis. We conclude that let-7i-5p plays an essential role in regulating cell shape and tissue elasticity, cell migration, cell morphology and cytoskeleton, and could serve as a potential target for clinical treatment of urethral stricture patients.https://www.frontiersin.org/article/10.3389/fbioe.2020.00428/fulllet-7i-5pmicroRNAcell migrationcell morphologyfibroblast |
| spellingShingle | Kaile Zhang Kaile Zhang Ranxin Yang Ranxin Yang Jun Chen Jun Chen Er Qi Shukui Zhou Ying Wang Qiang Fu Qiang Fu Rong Chen Rong Chen Xiaolan Fang Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts let-7i-5p microRNA cell migration cell morphology fibroblast |
| title | Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts |
| title_full | Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts |
| title_fullStr | Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts |
| title_full_unstemmed | Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts |
| title_short | Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts |
| title_sort | let 7i 5p regulation of cell morphology and migration through distinct signaling pathways in normal and pathogenic urethral fibroblasts |
| topic | let-7i-5p microRNA cell migration cell morphology fibroblast |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00428/full |
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