EVALUATION OF THE CYTOKINE STATUS IN PATIENTS WITH DIFFERENT VARIANTS OF THE COURSE OF HPV INFECTION

• Published in: Кубанский научный медицинский вестник
• Main Authors: E. V. KOLESNIKOVA, G. A. PENZHOYAN, V. A. ZHAROV, S. V. STOROZHUK
• Format: Article
• Language: Russian
• Published: Ministry of Healthcare of the Russian Federation. “Kuban State Medical University” 2018-10-01
• Subjects: cytokines; pvi; immunity; cervical pathology
• Online Access: https://ksma.elpub.ru/jour/article/view/1297

Aim. To evaluate the performance of some pro-inflammatory and anti-inflammatory cytokines in patients with various forms of  papillomavirus infection (PVI), to conduct a clinical evaluation of the  effective application of the drug containing a complex of sodium deoxyribonucleate with iron.Materials and methods. The examination of women with a latent course of PVI and a mild case of intraepithelial lesions of the cervix  L-SIL (Lowgrade Squamous Intraepitelial Lesions) was conducted  using a liquid-based Cytology ThinPrepR, colposcopy, biopsy of  cervix, detection of HPV 16 and 18 in the cervical smear by DNA  techniques (PCR in real time). Also, cytokines (IFN-gamma, IL-2, IL- 10, TNF-alpha) were quantified at the systemic level by the  immunoenzyme method. The repeated examination was carried out  after a course of treatment with a drug containing a complex of  sodium deoxyribonucleate with iron in 3 and 6 months.Results. After 6 months of HPV therapy PCR was not detected in 94% of patients with a latent course of PVI and 75% of patients with L-SIL. Patients with a latent course of PVI showed a significant  increase in the levels of IFNγ, TNF-α and IL-10 before the treatment.  Patients with L-SIL showed less significant increase in  IFNγ and TNF-α before the treatment, the levels of IL-10 and IL-2  had no significant deviations. After 3 months of the treatment  patients with a latent course of PVI showed a decrease in the  concentration of IFNγ, TNF-α and IL-10 to the indicators of  conditionally healthy women. Patients with L-SIL showed a slight  decrease in the concentration of TNF-α and a decrease in the  concentration of IL-10 to the indicators of conditionally healthy  women. The level of IFNγ and IL-2 remained almost at the same  level as before the treatment.Conclusion. The use of a drug containing a complex of sodium deoxyribonucleate with iron during a latent course of PVI can help to  prevent its transition to subclinical and clinical forms. The clinical  efficacy of the drug Ferrovir was confirmed, manifested in the fact  that 6 months after the treatment the virus DNA ceased to be  determined in 94% of cases in patients from the group with a latent  course of papillomavirus infection. There was a lack of immune  response to HPV in L-SIL, as well as less pronounced clinical efficacy of Ferrovir in this group. It can be assumed that a more pronounced clinical effect in the treatment of patients with L-SIL can be obtained  by using the complex therapy, including the appointment of the drug Ferrovir and destructive methods of treatment of the cervix or  an additional prescription of the targeted therapy, aimed at the treatment of already affected and altered cells of the cervical epithelium.