Effects of Testosterone on Analgesia in Formalin-Treated Mice

• Published in: Journal of Ardabil University of Medical Sciences
• Main Authors: Akram Alijani, Rahmatoolah Parandin, Namdar Yousofvand, SHahrbanoo Oryan
• Format: Article
• Language: Persian
• Published: Ardabil University of Medical Sciences 2018-04-01
• Subjects: Testosterone; Analgesia; Morphine; Spironolactone
• Online Access: http://jarums.arums.ac.ir/browse.php?a_code=A-10-607-97&slc_lang=en&sid=1
• ISSN: 2228-7280; 2228-7299

CT &nbsp; Background & objectives: So far, various reports have been presented on the relationship between sex hormones and gender-related differences in pain and analgesia in humans and laboratory animals. The&nbsp;purpose&nbsp;of this&nbsp;study&nbsp;was to&nbsp;investigate&nbsp;the&nbsp;effect of testosterone hormone and spironolactone anti-androgen drug on morphine-induced analgesia in male mice using formalin test. Methods: In this study, 80 male mice were divided into 10 groups (N=8); normal saline (control), sesame seed oil (as testosterone solvent), testosterone (5 and 10 mg/kg body weight), spironolactone, morphine, sesame seed oil + morphine, testosterone (5 and 10 mg/ kg body weight) + morphine and spironolactone + morphine. Formalin test was performed in all the mice, and data were analyzed by one-way ANOVA. Results: The results showed that sesame seed oil + morphine (p<0.001), morphine (p<0.001), testosterone (5 mg/kg) + morphine (p<0.01) and testosterone (10 mg/kg) + morphine (p<0.001) significantly reduced acute &nbsp;pain, and testosterone (5 mg/kg) (p<0.05), testosterone (10 mg/kg) (p<0.01), sesame seed oil + morphine (p<0.001), morphine (p<0.001), testosterone (5 mg/kg) + morphine (p<0.001) and testosterone (10 mg/kg) + morphine (p<0.001) significantly reduced chronic pain compared with control group. Spironolactone had no effect on pain relief in the presence and absence of morphine compared to control group. Conclusions: It can be concluded that testosterone has analgesic effects on the chronic phase of the pain. On the other hand, spironolactone may have hyperalgesic effects due to its anti-androgenic properties.