Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy
Dermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotizing myopathy (IMNM), and inclusion body myositis (IBM) are four major types of idiopathic inflammatory myopathy (IIM). Muscle biopsies from each type of IIM have unique transcriptomic profiles. MicroRNAs (miRNAs) target mess...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2073-4409/12/17/2198 |
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| author | Sandra Muñoz-Braceras Iago Pinal-Fernandez Maria Casal-Dominguez Katherine Pak José César Milisenda Shajia Lu Massimo Gadina Faiza Naz Gustavo Gutierrez-Cruz Stefania Dell’Orso Jiram Torres-Ruiz Josep Maria Grau-Junyent Albert Selva-O’Callaghan Julie J. Paik Jemima Albayda Lisa Christopher-Stine Thomas E. Lloyd Andrea M. Corse Andrew L. Mammen |
| author_facet | Sandra Muñoz-Braceras Iago Pinal-Fernandez Maria Casal-Dominguez Katherine Pak José César Milisenda Shajia Lu Massimo Gadina Faiza Naz Gustavo Gutierrez-Cruz Stefania Dell’Orso Jiram Torres-Ruiz Josep Maria Grau-Junyent Albert Selva-O’Callaghan Julie J. Paik Jemima Albayda Lisa Christopher-Stine Thomas E. Lloyd Andrea M. Corse Andrew L. Mammen |
| author_sort | Sandra Muñoz-Braceras |
| collection | DOAJ |
| container_title | Cells |
| description | Dermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotizing myopathy (IMNM), and inclusion body myositis (IBM) are four major types of idiopathic inflammatory myopathy (IIM). Muscle biopsies from each type of IIM have unique transcriptomic profiles. MicroRNAs (miRNAs) target messenger RNAs (mRNAs), thereby regulating their expression and modulating transcriptomic profiles. In this study, 18 DM, 12 IMNM, 6 AS, 6 IBM, and 6 histologically normal muscle biopsies underwent miRNA profiling using the NanoString nCounter system. Eleven miRNAs were exclusively differentially expressed in DM compared to controls, seven miRNAs were only differentially expressed in AS, and nine miRNAs were specifically upregulated in IBM. No differentially expressed miRNAs were identified in IMNM. We also analyzed miRNA-mRNA associations to identify putative targets of differentially expressed miRNAs. In DM and AS, these were predominantly related to inflammation and cell cycle progression. Moreover, our analysis showed an association between miR-30a-3p, miR-30e-3p, and miR-199b-5p downregulation in DM and the upregulation of target genes induced by type I interferon. In conclusion, we show that muscle biopsies from DM, AS, and IBM patients have unique miRNA signatures and that these miRNAs might play a role in regulating the expression of genes known to be involved in IIM pathogenesis. |
| format | Article |
| id | doaj-art-430bca4e170c4fd7ba24d5229acce44e |
| institution | Directory of Open Access Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-430bca4e170c4fd7ba24d5229acce44e2025-08-19T22:38:55ZengMDPI AGCells2073-44092023-09-011217219810.3390/cells12172198Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory MyopathySandra Muñoz-Braceras0Iago Pinal-Fernandez1Maria Casal-Dominguez2Katherine Pak3José César Milisenda4Shajia Lu5Massimo Gadina6Faiza Naz7Gustavo Gutierrez-Cruz8Stefania Dell’Orso9Jiram Torres-Ruiz10Josep Maria Grau-Junyent11Albert Selva-O’Callaghan12Julie J. Paik13Jemima Albayda14Lisa Christopher-Stine15Thomas E. Lloyd16Andrea M. Corse17Andrew L. Mammen18Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAMuscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAMuscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAMuscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAMuscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USATranslational Immunology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USATranslational Immunology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAGenomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAMuscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAMuscle Research Unit, Internal Medicine Service, Hospital Clinic de Barcelona, 08036 Barcelona, SpainSystemic Autoimmune Diseases Unit, Vall d’Hebron General Hospital, Universitat Autònoma de Barcelona, 08035 Barcelona, SpainDivision of Rheumatology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USADivision of Rheumatology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USAMuscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USADermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotizing myopathy (IMNM), and inclusion body myositis (IBM) are four major types of idiopathic inflammatory myopathy (IIM). Muscle biopsies from each type of IIM have unique transcriptomic profiles. MicroRNAs (miRNAs) target messenger RNAs (mRNAs), thereby regulating their expression and modulating transcriptomic profiles. In this study, 18 DM, 12 IMNM, 6 AS, 6 IBM, and 6 histologically normal muscle biopsies underwent miRNA profiling using the NanoString nCounter system. Eleven miRNAs were exclusively differentially expressed in DM compared to controls, seven miRNAs were only differentially expressed in AS, and nine miRNAs were specifically upregulated in IBM. No differentially expressed miRNAs were identified in IMNM. We also analyzed miRNA-mRNA associations to identify putative targets of differentially expressed miRNAs. In DM and AS, these were predominantly related to inflammation and cell cycle progression. Moreover, our analysis showed an association between miR-30a-3p, miR-30e-3p, and miR-199b-5p downregulation in DM and the upregulation of target genes induced by type I interferon. In conclusion, we show that muscle biopsies from DM, AS, and IBM patients have unique miRNA signatures and that these miRNAs might play a role in regulating the expression of genes known to be involved in IIM pathogenesis.https://www.mdpi.com/2073-4409/12/17/2198inflammatory myopathiesmyositismiRNANanoStringnCounter |
| spellingShingle | Sandra Muñoz-Braceras Iago Pinal-Fernandez Maria Casal-Dominguez Katherine Pak José César Milisenda Shajia Lu Massimo Gadina Faiza Naz Gustavo Gutierrez-Cruz Stefania Dell’Orso Jiram Torres-Ruiz Josep Maria Grau-Junyent Albert Selva-O’Callaghan Julie J. Paik Jemima Albayda Lisa Christopher-Stine Thomas E. Lloyd Andrea M. Corse Andrew L. Mammen Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy inflammatory myopathies myositis miRNA NanoString nCounter |
| title | Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy |
| title_full | Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy |
| title_fullStr | Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy |
| title_full_unstemmed | Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy |
| title_short | Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy |
| title_sort | identification of unique microrna profiles in different types of idiopathic inflammatory myopathy |
| topic | inflammatory myopathies myositis miRNA NanoString nCounter |
| url | https://www.mdpi.com/2073-4409/12/17/2198 |
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