<it>In vitro </it>cytotoxicity of <it>Strobilanthes crispus </it>ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell

• Published in: BMC Complementary and Alternative Medicine
• Main Authors: Chong Hueh, Rahmat Asmah, Yeap Swee, Md Akim Abdah, Alitheen Noorjahan, Othman Fauziah, Gwendoline-Ee Cheng
• Format: Article
• Language: English
• Published: BMC 2012-04-01
• Subjects: <it>Strobilanthes crispus</it>; MCF-7; Apoptosis; p53
• Online Access: http://www.biomedcentral.com/1472-6882/12/35
• ISSN: 1472-6882

<p>Abstract</p> <p>Background</p> <p><it>Strobilanthes crispus </it>has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of <it>S. crispus </it>is still unclear.</p> <p>Results</p> <p><it>Strobilanthes cripus </it>was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC₅₀of <it>S. crispus </it>ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.</p> <p>Conclusion</p> <p>Our findings suggest that <it>S. crispus </it>ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.</p>