Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis

• Published in: Folia Histochemica et Cytobiologica
• Main Authors: Halina Kemona, Joanna Matowicka-Karna, Maciej Kralisz
• Format: Article
• Language: English
• Published: Via Medica 2011-07-01
• Subjects: giardiosis; IL-5; IL-6; IL-13; TNF-alpha; IFN-gamma; NO
• Online Access: http://www.fhc.viamedica.pl/darmowy_pdf.phtml?indeks=2&indeks_art=1593
• ISSN: 0239-8508; 1897-5631

The current study aims to determine the involvement of cellular responses in combating Giardiaintestinalis invasion. The study group consisted of 44 women and 18 men, aged 18–72 years, infected withG. intestinalis. The diagnosis was established based on laboratory investigations (examination of stool, choloscopy,GSA-65). Blood for analysis was collected before antiparasitic treatment and two weeks after treatment termination.The control group consisted of 22 women and 18 men aged 20–45 years. The serum concentrations ofIL-5, IL-6, IL-13, TNF, IFN-g were assayed using a set of Quantikine human. The concentrations of NO in theserum were determined using a set of Total Nitric Oxide Assay. Patients infected with G. intestinalis showeda statistically significant increase in the levels of NO, IFN-g and IL-13. Even the antiparasitic treatment applieddid not reduce the levels of these parameters and only caused a rise in IL-6. Our study showed a lack of acuteinflammatory state in the course of G. intestinalis infection.