Effects of the preservation medium and storage duration of domestic cat ovaries on the maturational and developmental competence of oocytes in vitro

• Published in: The Journal of Reproduction and Development
• Main Authors: Takumi YOSHIDA, Md Emtiaj ALAM, Keisuke HANAFUSA, Yasunori TSUJIMOTO, Masaya TSUKAMOTO, Ryoji KANEGI, Toshio INABA, Kikuya SUGIURA, Shingo HATOYA
• Format: Article
• Language: English
• Published: The Society for Reproduction and Development 2022-01-01
• Subjects: blastocyst; feline oocyte; in vitro culture; in vitro maturation; preservation
• Online Access: https://www.jstage.jst.go.jp/article/jrd/68/2/68_2021-084/_pdf/-char/en
• ISSN: 0916-8818; 1348-4400

We examined the effectiveness of saline, Euro-Collins solution (EC), and ET-Kyoto solution (ET-K) as preservation media for the cold storage of feline ovaries. Ovaries were maintained in these media at 4°C for 24, 48, or 72 h until oocyte retrieval. The ET-K group exhibited a higher oocyte maturation rate than the saline group after 72 h of storage. Moreover, ET-K could sustain the competence of the feline oocytes to cleave after 48 h, and the morula formation rate of the ET-K group was higher than that of the other groups after 24 and 48 h. Furthermore, the ET-K group exhibited a higher blastocyst formation rate than the other groups after storage for 24 h, and only ET-K retained the developmental competence in blastocysts after 48 h of storage. In addition, regarding the cell numbers of the blastocysts, there was no significant difference among the tested groups. In conclusion, our results indicate that ET-K is a suitable preservation medium for feline ovaries.