Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus

The aim of this study was to evaluate the influence of methylglyoxal (MG) on organogenesis and regeneration of tobacco (Nicotiana tabacum L.) plants from callus in media containing glycine or succinate. The best improvement in shoot proliferation and shoot length was obtained in the medium supplemen...

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Bibliographic Details
Published in:Biologia Plantarum
Main Authors: A. Ray, S. Ray, S. Mukhopadhyay, M. Ray
Format: Article
Language:English
Published: Institute of Experimental Botany of the Czech Academy of Sciences 2013-06-01
Subjects:
δ
-aminolevulinic acid
benzyladenine
chlorophyll
regeneration
tobacco
Online Access:https://bp.ueb.cas.cz/artkey/bpl-201302-0003_Methylglyoxal-with-glycine-or-succinate-enhances-differentiation-and-shoot-morphogenesis-in-Nicotia.php
Description
Summary:The aim of this study was to evaluate the influence of methylglyoxal (MG) on organogenesis and regeneration of tobacco (Nicotiana tabacum L.) plants from callus in media containing glycine or succinate. The best improvement in shoot proliferation and shoot length was obtained in the medium supplemented with 0.1 mM MG and 0.5 mM glycine or 0.25 mM succinate. The histological studies showed vigorous development of corm like structures and shoot organogenesis from callus tissues cultured in MG supplemented media. Biochemical studies also revealed higher content of δ-aminolaevulinic acid (a precursor of chlorophyll) and of chlorophyll.
ISSN:1573-8264

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