| Summary: | Objective To construct human TNC expression plasmid for the investigation of the role of TNC in pancreatic cancer metastasis by using pancreatic cancer cell strain with stable over-expression of TNC. Methods TNC expression plasmid was constructed by unique primer design method, and then pancreatic cancer cell strain PANC1 with stable TNC expression were obtained by transfection and G418 screening. RT-qPCR and Western blot examined the expression of TNC. Transwell and Western blot were used to detecte the effects of TNC on cell migration, invasion and EMT-related molecules. Results TNC plasmid was successfully constructed. TNC promoted cell migration and invasion(P＜0.05), decreased epithelial marker E-cadherin expression and upregulated mesenchymal marker N-cadherin expression (P＜0.05). Conclusions The TNC expression plasmid and TNC stable expression pancreatic cancer cell strain may be used to explore the biological function of TNC and the pathogenesis of pancreatic cancer.
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