Next-Generation Pathology Using Multiplexed Immunohistochemistry: Mapping Tissue Architecture at Single-Cell Level

Single-cell omics aim at charting the different types and properties of all cells in the human body in health and disease. Over the past years, myriads of cellular phenotypes have been defined by methods that mostly required cells to be dissociated and removed from their original microenvironment, t...

Full description

Bibliographic Details
Published in:	Frontiers in Oncology
Main Authors:	Francesca Maria Bosisio, Yannick Van Herck, Julie Messiaen, Maddalena Maria Bolognesi, Lukas Marcelis, Matthias Van Haele, Giorgio Cattoretti, Asier Antoranz, Frederik De Smet
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022-07-01
Subjects:	multiplexed immunofluorescencence and immunohistochemistry spatial profiling single-cell ‘omics tissue architecture analysis methods for spatial profiling
Online Access:	https://www.frontiersin.org/articles/10.3389/fonc.2022.918900/full

Similar Items

Quantitative multiplex immunohistochemistry with colorimetric staining (QUIVER) may still benefit from MILAN
by: Maddalena M. Bolognesi, et al.
Published: (2023-06-01)

Multiplexed Immunohistochemistry and Digital Pathology as the Foundation for Next-Generation Pathology in Melanoma: Methodological Comparison and Future Clinical Applications
by: Yannick Van Herck, et al.
Published: (2021-03-01)

The tumor micro-environment in pediatric glioma: friend or foe?
by: Julie Messiaen, et al.
Published: (2023-10-01)

BRAQUE: Bayesian Reduction for Amplified Quantization in UMAP Embedding
by: Lorenzo Dall’Olio, et al.
Published: (2023-02-01)

Antibodies validated for routinely processed tissues stain frozen sections unpredictably
by: Maddalena M Bolognesi, et al.
Published: (2021-03-01)

Novel Chromogens for Immunohistochemistry in Spatial Biology
by: Bipin Gupta, et al.
Published: (2024-05-01)

Multiplex imaging analysis of the tumor immune microenvironment for guiding precision immunotherapy
by: Feng Liu, et al.
Published: (2025-07-01)

The future of immunohistochemistry: advancing precision medicine through artificial intelligence-driven spatial proteomics and multiplexed diagnostic technologies
by: Ruby Dhar, et al.
Published: (2025-08-01)

Multiplexed digital spatial profiling of invasive breast tumors from Black and White women
by: Angela R. Omilian, et al.
Published: (2022-01-01)

Immune contexture analysis in immuno‐oncology: applications and challenges of multiplex fluorescent immunohistochemistry
by: Reshma Shakya, et al.
Published: (2020-01-01)

RUNX2 as a novel biomarker for early identification of patients progressing to advanced-stage mycosis fungoides
by: Maria Danielsen, et al.
Published: (2024-10-01)

Multiplexed Digital Spatial Protein Profiling Reveals Distinct Phenotypes of Mononuclear Phagocytes in Livers with Advanced Fibrosis
by: Jaejun Lee, et al.
Published: (2022-10-01)

Spatial modelling of the tumor microenvironment from multiplex immunofluorescence images: methods and applications
by: Gayatri Kumar, et al.
Published: (2023-12-01)

Recent Progress in Multiplexed Single-Photon Sources
by: Peter Adam, et al.
Published: (2024-12-01)

The normal and fibrotic mouse lung classified by spatial proteomic analysis
by: Roberta Ciccimarra, et al.
Published: (2022-05-01)

Dual-Wavelength Polarization Multifunction Metalens Based on Spatial Multiplexing
by: Xiangshuo Shang, et al.
Published: (2025-01-01)

Spatial Transcriptomic Technologies
by: Tsai-Ying Chen, et al.
Published: (2023-08-01)

Generation of Photon Orbital Angular Momentum and Its Application in Space Division Multiplexing
by: Temitope M. Olaleye, et al.
Published: (2023-06-01)

A Sample-Sparing Multiplexed ADCP Assay
by: Audrey L. Butler, et al.
Published: (2019-08-01)

The Multiband over Spatial Division Multiplexing Sliceable Transceiver for Future Optical Networks
by: Laia Nadal, et al.
Published: (2023-11-01)

Unraveling mitotic protein networks by 3D multiplexed epitope drug screening
by: Lorenz J Maier, et al.
Published: (2018-08-01)

Dielectric metalenses at long-wave infrared wavelengths: Multiplexing and spectroscope
by: Feng Tang, et al.
Published: (2020-09-01)

Photonics Breakthroughs 2024: Multidimensional Integrated (de)Multiplexers for Optical Fiber Communications
by: Xianyi Feng, et al.
Published: (2025-01-01)

Spatio-Temporal Analysis and Clinical-Epidemiological Characterization of Visceral Leishmaniasis in Maranhão, Brazil, from 2009 to 2020
by: Carolina Azevedo Amaral, et al.
Published: (2024-04-01)

Think Outside the Fiber: Imaging Amplifier for Space-Multiplexed Optical Transmission
by: Ibrahim Ozdur, et al.
Published: (2012-01-01)

Spreading of localized attacks in spatial multiplex networks
by: Dana Vaknin, et al.
Published: (2017-01-01)

Overview of Multiplex Immunohistochemistry and Immunofluorescence Techniques  in the Lung Cancer Immunotherapy
by: Wenjia SUN, et al.
Published: (2021-01-01)

Multiplex network reconstruction for the coupled spatial diffusion of infodemic and pandemic of COVID-19
by: Xiaoqi Zhang, et al.
Published: (2021-04-01)

Robust dimethyl‐based multiplex‐DIA doubles single‐cell proteome depth via a reference channel
by: Marvin Thielert, et al.
Published: (2023-09-01)

A Fading Suppression Method for Φ-OTDR Systems Based on Multi-Domain Multiplexing
by: Shuai Tong, et al.
Published: (2025-04-01)

ELIMINATOR: essentiality analysis using multisystem networks and integer programming
by: Asier Antoranz, et al.
Published: (2022-08-01)

A living laboratory approach in the design of the user requirements of a spatial information platform
by: Maria Coetzee († 2 November 2014), et al.
Published: (2014-05-01)

Characterization of the tumor microenvironment and identification of spatially predictive biomarkers associated with beneficial neoadjuvant chemoradiotherapy in locally advanced rectal cancer
by: Shifen Zhang, et al.
Published: (2023-11-01)

PENGUIN: A rapid and efficient image preprocessing tool for multiplexed spatial proteomics
by: A.M. Sequeira, et al.
Published: (2024-12-01)

Real-Time Spatial-Division Multiplexing Transmission with Commercial 400 Gb/s Transponders
by: Yuyang Gao, et al.
Published: (2024-03-01)

Spatial Mode Division Multiplexing of Free-Space Optical Communications Using a Pair of Multiplane Light Converters and a Micromirror Array for Turbulence Emulation
by: David Benton, et al.
Published: (2024-03-01)

Topography of immune cell infiltration in different stages of coronary atherosclerosis revealed by multiplex immunohistochemistry
by: Kimberley R.G. Cortenbach, et al.
Published: (2023-02-01)

Spatially-resolved proteomics and transcriptomics: An emerging digital spatial profiling approach for tumor microenvironment
by: Wang Nan, et al.
Published: (2021-01-01)

Single-Shot Sub-Picosecond Ultrafast Microscopic Imaging Utilizing Spatial-Frequency Multiplexing for Ultrafast Laser-Induced Plasma Visualization
by: Hang Li, et al.
Published: (2025-09-01)

Performance Enhancement of an Orbital-Angular-Momentum-Multiplexed Free-Space Optical Link Under Atmospheric Turbulence Effects Using Spatial-Mode Multiplexing and Hybrid Diversity Based on Adaptive MIMO Equalization
by: Bedir Bedir Yousif, et al.
Published: (2019-01-01)