Effects of Workers Exposure to Nanoparticles Studied by NMR Metabolomics

• الحاوية / القاعدة: Applied Sciences
• المؤلفون الرئيسيون: Štěpán Horník, Lenka Michálková, Jan Sýkora, Vladimír Ždímal, Štěpánka Vlčková, Štěpánka Dvořáčková, Daniela Pelclová
• التنسيق: مقال
• اللغة: الإنجليزية
• منشور في: MDPI AG 2021-07-01
• الموضوعات: NMR metabolomics; human plasma; exhaled breath condensate; nanoparticles exposure
• الوصول للمادة أونلاين: https://www.mdpi.com/2076-3417/11/14/6601

In this study, the effects of occupational exposure to nanoparticles (NPs) were studied by NMR metabolomics. Exhaled breath condensate (EBC) and blood plasma samples were obtained from a research nanoparticles-processing unit at a national research university. The samples were taken from three groups of subjects: samples from workers exposed to nanoparticles collected before and after shift, and from controls not exposed to NPs. Altogether, 60 ¹H NMR spectra of exhaled breath condensate (EBC) samples and 60 ¹H NMR spectra of blood plasma samples were analysed, 20 in each group. The metabolites identified together with binning data were subjected to multivariate statistical analysis, which provided clear discrimination of the groups studied. Statistically significant metabolites responsible for group separation served as a foundation for analysis of impaired metabolic pathways. It was found that the acute effect of NPs exposure is mainly reflected in the pathways related to the production of antioxidants and other protective species, while the chronic effect is manifested mainly in the alteration of glutamine and glutamate metabolism, and the purine metabolism pathway.