Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma Patients
Non-high-risk (non-HR) neuroblastoma (NB) patients have excellent outcomes, with more than a 90% survival rate, whereas HR NB patients expect less than a 50% survival rate. Metastatic disease is the principal cause of death among both non-HR and HR NB patients. Previous studies have reported the sig...
| 出版年: | Biology |
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| 主要な著者: | , , , , , , , , , , , , , , , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
MDPI AG
2024-05-01
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| オンライン･アクセス: | https://www.mdpi.com/2079-7737/13/5/345 |
| _version_ | 1850548151686004736 |
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| author | Naoko Nakatani Kaung Htet Nay Win Cho Yee Mon Tomoko Fujikawa Suguru Uemura Atsuro Saito Toshiaki Ishida Takeshi Mori Daiichiro Hasegawa Yoshiyuki Kosaka Shotaro Inoue Akihiro Nishimura Nanako Nino Akihiro Tamura Nobuyuki Yamamoto Kandai Nozu Noriyuki Nishimura |
| author_facet | Naoko Nakatani Kaung Htet Nay Win Cho Yee Mon Tomoko Fujikawa Suguru Uemura Atsuro Saito Toshiaki Ishida Takeshi Mori Daiichiro Hasegawa Yoshiyuki Kosaka Shotaro Inoue Akihiro Nishimura Nanako Nino Akihiro Tamura Nobuyuki Yamamoto Kandai Nozu Noriyuki Nishimura |
| author_sort | Naoko Nakatani |
| collection | DOAJ |
| container_title | Biology |
| description | Non-high-risk (non-HR) neuroblastoma (NB) patients have excellent outcomes, with more than a 90% survival rate, whereas HR NB patients expect less than a 50% survival rate. Metastatic disease is the principal cause of death among both non-HR and HR NB patients. Previous studies have reported the significant but limited prognostic value of quantitative PCR (qPCR)-based assays, measuring overlapping but different sets of neuroblastoma-associated mRNAs (NB-mRNAs), to detect metastatic disease in both non-HR and HR patient samples. A droplet digital PCR (ddPCR)-based assay measuring seven NB-mRNAs (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNAs) was recently developed and exhibited a better prognostic value for HR patient samples than qPCR-based assays. However, it remained to be tested on non-HR patient samples. In the present study, we employed the ddPCR-based assay to study peripheral blood (PB) and bone marrow (BM) samples collected at diagnosis from eight non-HR and eleven HR cases and characterized the expression profiles of NB-mRNAs. The most highly expressed NB-mRNAs in PB and BM differed between non-HR and HR cases, with the CRMP1 mRNA being predominant in non-HR cases and the GAP43 mRNA in HR cases. The levels of NB-mRNAs in PB and BM were 5 to 1000 times lower in non-HR cases than in HR cases. The PB to BM ratio of NB-mRNAs was 10 to 100 times higher in non-HR cases compared to HR cases. The present case series suggests that non-HR and HR NB patients have the distinct expression profiles of NB-mRNAs in their PB and BM. |
| format | Article |
| id | doaj-art-4f520bdc7b0e4e60a8476e9f07f04f08 |
| institution | Directory of Open Access Journals |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-4f520bdc7b0e4e60a8476e9f07f04f082025-08-19T22:36:50ZengMDPI AGBiology2079-77372024-05-0113534510.3390/biology13050345Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma PatientsNaoko Nakatani0Kaung Htet Nay Win1Cho Yee Mon2Tomoko Fujikawa3Suguru Uemura4Atsuro Saito5Toshiaki Ishida6Takeshi Mori7Daiichiro Hasegawa8Yoshiyuki Kosaka9Shotaro Inoue10Akihiro Nishimura11Nanako Nino12Akihiro Tamura13Nobuyuki Yamamoto14Kandai Nozu15Noriyuki Nishimura16Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Public Health, Kobe University Graduate School of Health Science, Kobe 654-0142, JapanDepartment of Public Health, Kobe University Graduate School of Health Science, Kobe 654-0142, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Hematology/Oncology, Kobe Children’s Hospital, Kobe 650-0047, JapanDepartment of Hematology/Oncology, Kobe Children’s Hospital, Kobe 650-0047, JapanDepartment of Hematology/Oncology, Kobe Children’s Hospital, Kobe 650-0047, JapanDepartment of Hematology/Oncology, Kobe Children’s Hospital, Kobe 650-0047, JapanDepartment of Hematology/Oncology, Kobe Children’s Hospital, Kobe 650-0047, JapanDepartment of Hematology/Oncology, Kobe Children’s Hospital, Kobe 650-0047, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Hematology/Oncology, Kobe Children’s Hospital, Kobe 650-0047, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Public Health, Kobe University Graduate School of Health Science, Kobe 654-0142, JapanNon-high-risk (non-HR) neuroblastoma (NB) patients have excellent outcomes, with more than a 90% survival rate, whereas HR NB patients expect less than a 50% survival rate. Metastatic disease is the principal cause of death among both non-HR and HR NB patients. Previous studies have reported the significant but limited prognostic value of quantitative PCR (qPCR)-based assays, measuring overlapping but different sets of neuroblastoma-associated mRNAs (NB-mRNAs), to detect metastatic disease in both non-HR and HR patient samples. A droplet digital PCR (ddPCR)-based assay measuring seven NB-mRNAs (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNAs) was recently developed and exhibited a better prognostic value for HR patient samples than qPCR-based assays. However, it remained to be tested on non-HR patient samples. In the present study, we employed the ddPCR-based assay to study peripheral blood (PB) and bone marrow (BM) samples collected at diagnosis from eight non-HR and eleven HR cases and characterized the expression profiles of NB-mRNAs. The most highly expressed NB-mRNAs in PB and BM differed between non-HR and HR cases, with the CRMP1 mRNA being predominant in non-HR cases and the GAP43 mRNA in HR cases. The levels of NB-mRNAs in PB and BM were 5 to 1000 times lower in non-HR cases than in HR cases. The PB to BM ratio of NB-mRNAs was 10 to 100 times higher in non-HR cases compared to HR cases. The present case series suggests that non-HR and HR NB patients have the distinct expression profiles of NB-mRNAs in their PB and BM.https://www.mdpi.com/2079-7737/13/5/345high-risk (HR) neuroblastoma (NB)non-high-risk (non-HR) neuroblastoma (NB)neuroblastoma-associated mRNAs (NB-mRNAs)peripheral blood (PB)bone marrow (BM) |
| spellingShingle | Naoko Nakatani Kaung Htet Nay Win Cho Yee Mon Tomoko Fujikawa Suguru Uemura Atsuro Saito Toshiaki Ishida Takeshi Mori Daiichiro Hasegawa Yoshiyuki Kosaka Shotaro Inoue Akihiro Nishimura Nanako Nino Akihiro Tamura Nobuyuki Yamamoto Kandai Nozu Noriyuki Nishimura Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma Patients high-risk (HR) neuroblastoma (NB) non-high-risk (non-HR) neuroblastoma (NB) neuroblastoma-associated mRNAs (NB-mRNAs) peripheral blood (PB) bone marrow (BM) |
| title | Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma Patients |
| title_full | Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma Patients |
| title_fullStr | Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma Patients |
| title_full_unstemmed | Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma Patients |
| title_short | Distinct Expression Profiles of Neuroblastoma-Associated mRNAs in Peripheral Blood and Bone Marrow of Non-High-Risk and High-Risk Neuroblastoma Patients |
| title_sort | distinct expression profiles of neuroblastoma associated mrnas in peripheral blood and bone marrow of non high risk and high risk neuroblastoma patients |
| topic | high-risk (HR) neuroblastoma (NB) non-high-risk (non-HR) neuroblastoma (NB) neuroblastoma-associated mRNAs (NB-mRNAs) peripheral blood (PB) bone marrow (BM) |
| url | https://www.mdpi.com/2079-7737/13/5/345 |
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