SEROLOGICAL AND BIOLOGICAL ACTIVITY OF LIPOPOLYSACCHARIDE FROM Escherichia coli L-19

• Published in: Biotechnologia Acta
• Main Authors: L. D. Varbanets, E. L. Zdorovenko, Е. G. Garkavaya, O. S. Brovarskaya
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 2015-02-01
• Subjects: Escherichia coli lipopolysaccharide; toxicity; pyrogenicity; mitogenic activity
• Online Access: http://biotechnology.kiev.ua/images/storage/no1_2015/varbanets_1_2015_.pdf

The lipopolysaccharide from Escherichia coli L-19 was isolated, chemically identified and its nontoxicity but pyrogenicity were shown. The investigations of lipopolysaccharide influence on T- and B-lymphocytes indicated that it might be used as a mitogen in blasttransformation reactions since it was only in insignificant degree less active in comparison with the commercial one. It was shown that in reactions of Ouchterlony double immunodiffusion in agar LPS from E. coli L-19 exerted an activity of antigen in homological system. There were not established serological cross reactions between antiserum to heated E.coli L-19 cells and LPS from another of E.coli strain М-17 аnd also the representatives of Enterobacteriaceae species such as Budvicia aquatica, Rahnella aquatilis and Pragia fontium. These results indicate the absence of common antigenic determinants between the tested strains.