Tuning tRNAs for improved translation
Transfer RNAs have been extensively explored as the molecules that translate the genetic code into proteins. At this interface of genetics and biochemistry, tRNAs direct the efficiency of every major step of translation by interacting with a multitude of binding partners. However, due to the variabi...
| 出版年: | Frontiers in Genetics |
|---|---|
| 主要な著者: | , , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
Frontiers Media S.A.
2024-06-01
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| 主題: | |
| オンライン･アクセス: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1436860/full |
| _version_ | 1850100328441053184 |
|---|---|
| author | Joshua L. Weiss J. C. Decker Ariadna Bolano Natalie Krahn |
| author_facet | Joshua L. Weiss J. C. Decker Ariadna Bolano Natalie Krahn |
| author_sort | Joshua L. Weiss |
| collection | DOAJ |
| container_title | Frontiers in Genetics |
| description | Transfer RNAs have been extensively explored as the molecules that translate the genetic code into proteins. At this interface of genetics and biochemistry, tRNAs direct the efficiency of every major step of translation by interacting with a multitude of binding partners. However, due to the variability of tRNA sequences and the abundance of diverse post-transcriptional modifications, a guidebook linking tRNA sequences to specific translational outcomes has yet to be elucidated. Here, we review substantial efforts that have collectively uncovered tRNA engineering principles that can be used as a guide for the tuning of translation fidelity. These principles have allowed for the development of basic research, expansion of the genetic code with non-canonical amino acids, and tRNA therapeutics. |
| format | Article |
| id | doaj-art-52c8fa8a033a4c1780851731771bbf5d |
| institution | Directory of Open Access Journals |
| issn | 1664-8021 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| spelling | doaj-art-52c8fa8a033a4c1780851731771bbf5d2025-08-20T00:05:04ZengFrontiers Media S.A.Frontiers in Genetics1664-80212024-06-011510.3389/fgene.2024.14368601436860Tuning tRNAs for improved translationJoshua L. WeissJ. C. DeckerAriadna BolanoNatalie KrahnTransfer RNAs have been extensively explored as the molecules that translate the genetic code into proteins. At this interface of genetics and biochemistry, tRNAs direct the efficiency of every major step of translation by interacting with a multitude of binding partners. However, due to the variability of tRNA sequences and the abundance of diverse post-transcriptional modifications, a guidebook linking tRNA sequences to specific translational outcomes has yet to be elucidated. Here, we review substantial efforts that have collectively uncovered tRNA engineering principles that can be used as a guide for the tuning of translation fidelity. These principles have allowed for the development of basic research, expansion of the genetic code with non-canonical amino acids, and tRNA therapeutics.https://www.frontiersin.org/articles/10.3389/fgene.2024.1436860/fulltRNA engineeringsynthetic biologytranslationgenetic code expansionnoncanonical amino aciddirected evolution |
| spellingShingle | Joshua L. Weiss J. C. Decker Ariadna Bolano Natalie Krahn Tuning tRNAs for improved translation tRNA engineering synthetic biology translation genetic code expansion noncanonical amino acid directed evolution |
| title | Tuning tRNAs for improved translation |
| title_full | Tuning tRNAs for improved translation |
| title_fullStr | Tuning tRNAs for improved translation |
| title_full_unstemmed | Tuning tRNAs for improved translation |
| title_short | Tuning tRNAs for improved translation |
| title_sort | tuning trnas for improved translation |
| topic | tRNA engineering synthetic biology translation genetic code expansion noncanonical amino acid directed evolution |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1436860/full |
| work_keys_str_mv | AT joshualweiss tuningtrnasforimprovedtranslation AT jcdecker tuningtrnasforimprovedtranslation AT ariadnabolano tuningtrnasforimprovedtranslation AT nataliekrahn tuningtrnasforimprovedtranslation |