A highly efficient genetic transformation system for broccoli and subcellular localization

IntroductionAgrobacterium-mediated genetic transformation has been widely used for the identification of functional genes and regulatory and developmental mechanisms in plants. However, there are still some problems of low genetic transformation efficiency and high genotype dependence in cruciferous...

Full description

Bibliographic Details
Published in:	Frontiers in Plant Science
Main Authors:	Yongyu Zhao, Dongxu Yang, Yumei Liu, Fengqing Han, Zhansheng Li
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-03-01
Subjects:	broccoli agrobacterium CRISPR/Cas9 subcellular localization genetic transformation transgenic
Online Access:	https://www.frontiersin.org/articles/10.3389/fpls.2023.1091588/full

Similar Items

A high-efficiency PEG-Ca2+-mediated transient transformation system for broccoli protoplasts
by: Dongxu Yang, et al.
Published: (2022-12-01)

Exploring Agrobacterium-mediated genetic transformation methods and its applications in Lilium
by: Xinyue Fan, et al.
Published: (2024-08-01)

Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation
by: Zsófia Bánfalvi, et al.
Published: (2020-05-01)

Genetic Diversity and DNA Fingerprinting in Broccoli Carrying Multiple Clubroot Resistance Genes Based on SSR Markers
by: Qi Xie, et al.
Published: (2022-05-01)

Advances in Genetics and Molecular Breeding of Broccoli
by: Fengqing Han, et al.
Published: (2021-09-01)

Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation in citrus seeds and its application in gene functional analysis
by: Min Wang, et al.
Published: (2023-10-01)

Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea (Pisum sativum L.)
by: Guan Li, et al.
Published: (2023-02-01)

Development of genetic tools for the thermophilic filamentous fungus Thermoascus aurantiacus
by: Raphael Gabriel, et al.
Published: (2020-10-01)

A simple and efficient strategy to produce transgene-free gene edited plants in one generation using paraquat resistant 1 as a selection marker
by: Xiangjiu Kong, et al.
Published: (2023-01-01)

Construction and Identification of Cold Tolerance in Different Broccoli Cultivars at the Seedling Stage
by: Dongna Wen, et al.
Published: (2024-01-01)

Genetically modified foods: A critical review of their promise and problems
by: Chen Zhang, et al.
Published: (2016-09-01)

Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers
by: Jingjing Huang, et al.
Published: (2021-09-01)

Streamlined Agrobacterium rhizogenes-mediated hairy root transformation for efficient CRISPR/Cas9-based gene editing evaluation in diverse Citrullus cultivars
by: Yong Zhao, et al.
Published: (2025-03-01)

Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit
by: Pengwei Li, et al.
Published: (2024-01-01)

Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene editing analysis in cotton
by: Lili Zhou, et al.
Published: (2022-12-01)

Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR
by: Ming Li, et al.
Published: (2018-02-01)

Diversity of transgene integration and gene-editing events in wheat (Triticum aestivum L.) transgenic plants generated using Agrobacterium-mediated transformation
by: Louie Cris Lopos, et al.
Published: (2023-12-01)

Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.
by: Olha Mikolaivna Yaroshko, et al.
Published: (2018-12-01)

Enhancing Maize Transformation and Targeted Mutagenesis through the Assistance of Non-Integrating <i>Wus2</i> Vector
by: Minjeong Kang, et al.
Published: (2023-07-01)

Enabling genome editing in tropical maize lines through an improved, morphogenic regulator-assisted transformation protocol
by: José Hernandes-Lopes, et al.
Published: (2023-12-01)

Increasing of Amylopectin in Tetraploid Potato Desiree Cultivar (Solanum tuberosum L.) Via CRISPR/Cas9
by: Nermin Ali, et al.
Published: (2023-12-01)

Blocking drug efflux mechanisms facilitate genome engineering process in hypercellulolytic fungus, Penicillium funiculosum NCIM1228
by: Anmoldeep Randhawa, et al.
Published: (2021-01-01)

An Efficient System for Mesophyll Protoplast Isolation, Purification, and Transformation in Loquat: Studies on Fluorescent Marker Analysis and Subcellular Localization
by: Shuming Wang, et al.
Published: (2025-04-01)

Development of Protoplast-Based Gene Editing System for Areca Palm
by: Hao Nie, et al.
Published: (2025-03-01)

Identification and Evaluation of Thermotolerance in Broccoli Seedlings Based on a Multi-Trait Phenotyping System
by: Xuaner Li, et al.
Published: (2025-08-01)

<i>Agrobacterium rhizogenes-</i>Mediated Genetic Transformation and Establishment of CRISPR/Cas9 Genome-Editing Technology in <i>Limonium bicolor</i>
by: Qianqian Li, et al.
Published: (2023-08-01)

Transformation efficiency of Arabidopsis thaliana ecotypes with differential tolerance to submergence stress
by: Mayra Santiago-Velasco, et al.
Published: (2022-12-01)

Genetically Modified Rabbits for Cardiovascular Research
by: Jianglin Fan, et al.
Published: (2021-02-01)

The progress of genetic improvement in alfalfa (Medicago sativa L.)
by: Tanweer KUMAR, et al.
Published: (2018-06-01)

Development of Genetic Tools in Glucoamylase-Hyperproducing Industrial <i>Aspergillus niger</i> Strains
by: Dandan Liu, et al.
Published: (2022-09-01)

Designing A Transgenic Chicken: Applying New Approaches Toward A Promising Bioreactor
by: Salahadin Bahrami, et al.
Published: (2019-07-01)

Efficient Agrobacterium-mediated transformation and genome editing of Fagopyrum tataricum
by: Artur Pinski, et al.
Published: (2023-09-01)

Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update
by: Manjulatha Mekapogu, et al.
Published: (2023-11-01)

Editorial: Proceedings of ICPSBBB 2018 - 2nd International Conference on Plant Synthetic Biology, Bioengineering and Biotechnology
by: Henrik Vibe Scheller, et al.
Published: (2020-11-01)

Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines
by: Zhansheng Li, et al.
Published: (2022-02-01)

GENETICALLY MODIFIED FRUITS: THE SOLUTION OR A PROBLEM? [GENETICKY MODIFIKOVANÉ OVOCE: ŘEŠENÍ NEBO PROBLÉM?]
by: Michaela Burešová
Published: (2025-05-01)

Recent Progress in the Regeneration and Genetic Transformation System of Cucumber
by: Jihong Tan, et al.
Published: (2022-07-01)

Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses
by: Chunjia Li, et al.
Published: (2024-03-01)

CRISPR-based gene editing in plants: Focus on reagents and their delivery tools
by: Vida Ebrahimi, et al.
Published: (2024-06-01)

Genome-wide evaluation of gene editing outcomes using CRISPR/Cas9 in seed propagated Camelina sativa and vegetatively propagated Solanum tuberosum
by: Thilani B. Jayakody, et al.
Published: (2024-11-01)